AQ9511 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心

AQ9511 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名：AQ9511
Plasmid Integrated整合质粒：
Plasmid autonomous：
Prophage：λ+ λGM108A(rnh+ dnaQ::lacZ)
编号:ME9544
Genotype基因型:thyA708 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 deo-29 rnhA102 recA200 sfiA11 lexA71::Tn5 polA25::spc zih-35::Tn10 λGM108A(rnh+ dnaQ::lacZ)
Parent donor：λGM108A
Parent recipient:AQ8619
Sex：F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:OTHER METHODS λ infection
Latest marker:λGM108A(rnh+ dnaQ::lacZ)
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.

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