AQ9250 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心

AQ9250 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名：AQ9250
Plasmid Integrated整合质粒：
Plasmid autonomous：
Prophage：AQ8937
编号:ME9526
Genotype基因型:nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 tnaA::dTn10(Tc-s) zih-35::dTn10(Tc-s) ΔpolB::spc zac-3051::Tn10 dnaA5 lacZ118 metE90 trpA9605 lacI22 rpsL171 azi-9
Parent donor：AQ9239
Parent recipient:AQ8937
Sex：F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:Spc-r, ΔpolB
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.

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