AQ9077 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心

AQ9077 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名：AQ9077
Plasmid Integrated整合质粒：
Plasmid autonomous：
Prophage：
编号:ME9510
Genotype基因型:recB270(Ts) oriCdel-1071 zif90::pML31 metE46 trp-3 his-4 thi-1 galK2 tsx-3 ton-1 rpsL8 (or rpsL9) supE44 thyA zga::dTn10(Tc-s) rnhA339::cat lacY1 (or lacZ4) mtl-1 ara-9
Parent donor：AQ3634
Parent recipient:AQ8964
Sex：F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml).
Methods:TD./PI
Latest marker:Ts, recB270
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.

Supplier:
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