AQ5937 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心

AQ5937 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名：AQ5937
Plasmid Integrated整合质粒：
Plasmid autonomous：
Prophage：
编号:ME9361
Genotype基因型:xyl-5 mtl-1 lexA71::Tn5 tsx-33 argE3 thi-1 rpsL31 galK2 lac+ ilv(ts) supE44 Δ(gpt-proA)62 leuB6 recB21 thr-1 lac ara-14 recA441 sulA221 his-4 zga::Tn10
Parent donor：AQ5931
Parent recipient:AQ2125(JM441)
Sex：F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:UV-s, Rec-, recB21
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.


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