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AQ5931 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名:AQ5931
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9360
Genotype基因型:proB(or proC) trpA9605 his-29 ilv metB1 deoB(or deoC) zga::Tn10 recB21
Parent donor:AQ169
Parent recipient:AQ5786
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:UV-s, Rec-, recB21
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
Strain菌株名:AQ5931
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9360
Genotype基因型:proB(or proC) trpA9605 his-29 ilv metB1 deoB(or deoC) zga::Tn10 recB21
Parent donor:AQ169
Parent recipient:AQ5786
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:UV-s, Rec-, recB21
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
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