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AQ527 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名:AQ527
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9118
Genotype基因型:metE90 rin-15 recA200 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102
Parent donor:KL268
Parent recipient:AQ377
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:CROSS MUTAGENESIS/Others EMS treatment
Latest marker:rin-15
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
Strain菌株名:AQ527
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9118
Genotype基因型:metE90 rin-15 recA200 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102
Parent donor:KL268
Parent recipient:AQ377
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:CROSS MUTAGENESIS/Others EMS treatment
Latest marker:rin-15
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
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