Vibrio qinghaiensis sp. Q67 青海湖菌strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

Vibrio qinghaiensis sp. Q67 青海湖菌strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Biovector595402 Vibrio qinghaiensis sp. Q67 Vibrio qinghaiensis sp. Q67,1vial Storage:4℃

Of the methods for ecotoxicity assessment, bioassays using luminescent bacteria are widely applied in water toxicity tests for its advantages of good sensibility, reproducibility, flexibility and low cost (Parvez et al., 2006; Mendonça et al., 2009). The luminescent bacteria assays often show good correlations to toxicity bioassays using other flora and fauna such as algae, crustacean and fish (Girotti et al., 2008). As an alternative luminescent bacterium, Vibrio qinghaiensis sp. Q67 (abbreviated as “Q67”) has been isolated from the body surface of Cymnocypris przewalskii, one of the edible fish in Qinghai Province, China (Zhu et al., 1994). It is verified that Q67 has similar property to other luminescent bacteria in light emission and inhibition, and can be used for the bioassay under a freshwater environment which much benefits the toxicity tests in most cases (Ma et al., 1998). Nowadays, both bioassays using Photobacterium phosphoreum T3 spp. (marine strain) and Q67 are recommended as applicable methods for ecotoxicity tests.

      Culture medium:
KH2PO4 27. 2mg ,
Na2HPO4 • 12H2O 71. 6mg,
MgSO4 • 7H2O  0. 5g,
MgCl2 • 6H2O 1. 22g,
CaCl2 66.0 mg,
NaHCO3   2. 68g,
NaCl  3. 08g,
yeast extract paste 5. 0g,
tryptone 5. 0g,
glycerol 3. 0g,
distilled water 1000mL

adjust pH using 1mol / L NaOH to 8.5 ± 0.5
autoclave sterilization under 121 ° C, 20min

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