KOPN-8 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

KOPN-8 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
KOPN-8
Cat No.:
ACC552
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells were described in the literature to carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
single round rather small cells growing in suspension;
image

Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml in 12-well-plates with 20% FBS; maintain at ca. 0.5-1.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD38 +, CD80 -, CD138 -, HLA-DR +, cyIgG -, cyIgM +;
image

Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by PCR speciation and cytogenetics
Cytogenetics:
human hypodiploid karyotype with 4% polyploidy - 45(42-45)<2n>XX, -14, t(8;13)(q24;q21.2), t(11;19)(q23;p13), der(13)t(13;14)(p11;q11) - carries t(11;19) effecting KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion - matches published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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