BA/F3_Her2 A775_G776insYVMA激酶Kinase稳定表达细胞株-BioVector NTCC保藏中心
- 价 格：¥0
- 货 号：BA/F3_Her2 A775_G776insYVMA
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
BA/F3_Her2 A775_G776insYVMA激酶Kinase稳定表达细胞株-BioVector NTCC保藏中心
I. Introduction
Cell Line Name：
BA/F3_Her2 A775_G776insYVMA
Gene Synonyms:
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Host Cell:
Ba/F3
Stability: 16 passages
Application:
Anti-proliferation assay and PD assay
Freeze Medium:
90% FBS+10% DMSO
Complete Culture Medium:
RPMI-1640+10%FBS+1 ug/ml puromycin
Mycoplasma Status:
Negative
II.Background
ERBB2 (HER2) A775_G776insYVMA results in the insertion of four amino acids in the protein kinase domain of the Erbb2 (Her2) protein between amino acids 775 and 776 (UniProt.org). A775_G776insYVMA results in the same amino acid change as M774_A775insAYVM, which leads to constitutive phosphorylation of Erbb2 (Her2) and is transforming in cell culture (PMID: 17311002, PMID: 29967253).
III. Representative Data
1. Anti-proliferation assay
Figure 1. Anti-proliferation assay of two reference compounds on the BA/F3_Her2 A775_G776insYVMA Stable Cell Line
IV. Thawing
Thawing: Protocol
1. Remove the vial from liquid nitrogen tank and thaw cells quickly in a 37°C water-bath.
2. Just before the cells are completely thawed, decontaminate the outside of the vial with 70% ethanol and transfer the cells to a 15 ml centrifuge tube containing 9 ml of complete growth medium.
3. Pellet cells by centrifugation at 200 x g force for 5 min, and discard the medium.
4. Resuspend the cells in complete growth medium.
5. Add 10 ml of the cell suspension in a 10 cm dish.
6. Add promycin to a concentration of 1 μg/ml the following day.
Kinase细胞株
现代新药研发的关键首先是寻找，确定和制备药物作用靶点。在500多个已发现的药物靶点里中，GPCR，Ion Channel，Kinase使用的最为广泛。
激酶(kinase)是一类从高能供体分子（如ATP）转移磷酸基团到特定靶分子（底物）的酶；这一过程谓之磷酸化。许多肿瘤的发生是由某些与生长相关的“激酶”发生突变导致异常活化引起的，因而针对这些突变激酶的抑制剂能够有效抑制这些激酶的活性，从而达到抑制癌细胞增长的目的。
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
I. Introduction
Cell Line Name：
BA/F3_Her2 A775_G776insYVMA
Gene Synonyms:
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Host Cell:
Ba/F3
Stability: 16 passages
Application:
Anti-proliferation assay and PD assay
Freeze Medium:
90% FBS+10% DMSO
Complete Culture Medium:
RPMI-1640+10%FBS+1 ug/ml puromycin
Mycoplasma Status:
Negative
II.Background
ERBB2 (HER2) A775_G776insYVMA results in the insertion of four amino acids in the protein kinase domain of the Erbb2 (Her2) protein between amino acids 775 and 776 (UniProt.org). A775_G776insYVMA results in the same amino acid change as M774_A775insAYVM, which leads to constitutive phosphorylation of Erbb2 (Her2) and is transforming in cell culture (PMID: 17311002, PMID: 29967253).
III. Representative Data
1. Anti-proliferation assay
Figure 1. Anti-proliferation assay of two reference compounds on the BA/F3_Her2 A775_G776insYVMA Stable Cell Line
IV. Thawing
Thawing: Protocol
1. Remove the vial from liquid nitrogen tank and thaw cells quickly in a 37°C water-bath.
2. Just before the cells are completely thawed, decontaminate the outside of the vial with 70% ethanol and transfer the cells to a 15 ml centrifuge tube containing 9 ml of complete growth medium.
3. Pellet cells by centrifugation at 200 x g force for 5 min, and discard the medium.
4. Resuspend the cells in complete growth medium.
5. Add 10 ml of the cell suspension in a 10 cm dish.
6. Add promycin to a concentration of 1 μg/ml the following day.
Kinase细胞株
现代新药研发的关键首先是寻找，确定和制备药物作用靶点。在500多个已发现的药物靶点里中，GPCR，Ion Channel，Kinase使用的最为广泛。
激酶(kinase)是一类从高能供体分子（如ATP）转移磷酸基团到特定靶分子（底物）的酶；这一过程谓之磷酸化。许多肿瘤的发生是由某些与生长相关的“激酶”发生突变导致异常活化引起的，因而针对这些突变激酶的抑制剂能够有效抑制这些激酶的活性，从而达到抑制癌细胞增长的目的。
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
- 公告/新闻
