NOMO-1 cell line急性髓细胞性白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NOMO-1 cell line急性髓细胞性白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: NOMO-1 DSMZ no.: ACC 542Species: human (Homo sapiens)Cell type: acute myeloid leukemia Origin: established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11)(q23;p22) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration Reference(s): 14595, 15031, 14493, 15144, 16555Biosafety level: 1 Permissions and restrictions: A  DSMZ Cell Culture Data: Morphology:single round cells growing in suspension; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS Subculture: seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing cell growth may be retarded; maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 twice a week Incubation: at 37 °C with 5% CO2 Doubling time: ca. 35 hours Harvest: cell harvest of ca. 1.5-2 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO  DSMZ Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +; image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human by cytogenetics and recently by STR fingerprinting Cytogenetics: human hyperdiploid karyotype with 8% polyploidy - 46-47<2n>XX, +8, -13, +mar, add(7)(q32), der(9)del(9)(p11p13-21)t(9;11)(p22;q23), t(9;13)(q13;q11), der(11)t(9;11)(p22;q23) - carries reciprocal t(9;11) with rearrangement of KMT2A (MLL) - matches published karyotype Molec. Genetics: expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV
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