pEN11-PAGL BioVector NTCC质粒载体菌种细胞基因保藏中心

pEN11-PAGL BioVector NTCC质粒载体菌种细胞基因保藏中心
Plasmid pEN11-pagL was obtained by subcloning the pagL gene of Bordetella bronchiseptica from plasmid pPagL(Bb) (J.G., L. Steeghs, J. ten Hove, A. de Jong, P. van der Ley, and J.T., unpublished work) into pEN11 by using NdeI–AatII restriction. This plasmid was used to transform H44/76 L8 immunotype. Correct transformants were identified by PCR and the imp gene was subsequently inactivated as described above.
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