pGH00.0126质粒载体BioVector NTCC保藏中心

pGH00.0126,

Plasmid BioVector® 064257.

Gene/Insert:EGFP, neomycin phosphotransferase II

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation.

E12-omega is a strong constitutive promoter obtained from pE2113-GUS (Mitsuhara et al. 1996). The promoter consists of two directly repeated copies the 5’ upstream sequence of the cauliflower mosaic virus (CaMV) 35S promoter (-419 to -90), followed by one copy of the P35S, the 5’-upstream sequence of the CaMV 35S promoter (–90 to –1) and the 5’ Omega-untranslated sequence of the tobacco mosaic virus. The Omega sequence is known to enhance translation levels in plant, animal and E. coli. This chimeric promoter was shown to support very high protein expression levels (approx. 23-fold higher then the standard CaMV 35S promoter) based on high transcription and enhanced translation rates (Mitsuhara et al. 1996).

Note-there is a 2nd NPTII cassette under the NOS Promoter. Only 1 NPTII region mutation was corrected and that is noted in the GenBank record.

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