出品公司: | BioVector |
---|---|
载体名称: | pMAL-p5x |
质粒类型: | 大肠杆菌分泌型表达载体;蛋白表达与纯化 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | tac |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5752bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | MBP tag |
载体抗性: | Ampicillin |
筛选标记: | -- |
备注: | -- |
产品目录号: | N8109S |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pMAL-p5x大肠杆菌分泌型表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
- 价 格:¥9862
- 货 号:pMAL-p5x大肠杆菌分泌型表达载体
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pMAL-p5x大肠杆菌分泌型表达载体
载体质粒图谱和多克隆位点信息
载体简介
The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa.MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein).The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.
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