pET MBP His6 LIC cloning vector (2Cc-T) 原核表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pET MBP His6 LIC cloning vector (2Cc-T) 原核表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. For more details, see the MacroLab vector cloning manual.

The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z).

2Cc-T has a TEV-cleavable C-terminal MBP tag to enhance solubility, as well as a His6 tag to ease purification. The TEV site is created after performing LIC cloning described below.

To clone into this vector, add LIC v3 tags to the 5' end of your PCR primers.

Forward - 5'TTTAAGAAGGAGATATAGTTC(ATG)3'

Reverse - 5'GGATTGGAAGTAGAGGTTCTC3'

Linearize the plasmid with HpaI and gel purify.

When digesting the DNA with T4 polymerase, use dGTP for insert and dCTP for vector.
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