pET-27b(+)原核表达载体pET27b带有pelB外周质腔定位信号肽 BioVector NTCC质粒载体菌种细胞基因保藏中心

pET-27b(+)原核表达载体pET27b带有pelB外周质腔定位信号肽 BioVector NTCC质粒载体菌种细胞基因保藏中心

带有pelB外周质腔定位信号肽，C端HSV标签和His标签。

The pET-27b(+) vector (Cat. No. 69863-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance).Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer
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