铜绿假单胞菌(Bacteriump yocyaneum ATCC-27853) BioVector NTCC质粒载体菌种细胞基因保藏中心

铜绿假单胞菌(Bacteriump yocyaneum ATCC-27853) BioVector NTCC质粒载体菌种细胞基因保藏中心
Designation: Boston 41501
Deposited Name: Pseudomonas aeruginosa (Schroeter) Migula
Product Description: Used in media and susceptibility testing, evaluation of MuellerHinton
Agar, and as a
quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, MicroMedia,
MicroScan™, Roche
Diagnostics, and Sensititre products.
Medium
ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Propagation Procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or
1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
Two colony types may be observed. They have been individually characterized and found to be identical.
The predominant type is erose, rough, low convex, and approximately 2 mm in diameter. The secondary type
is entire, glistening, smooth, and small. This strain produces both fluorescein and pyocyanin pigments.
To prevent the proliferation of the second colony type, avoid passing this strain through broth for transfers. It
is recommended to wash a slant with phosphate buffer and use that to inoculate further agar plates and/or
agar flasks if needed.
Purified genomic DNA of this strain is available as ATCC ® 27853D5
™. Certified Reference Material of this
strain is available as ATCC® CRM27853
™.
Several Genbank accessions are available for this item:
1. Nucleotide (GenBank) : AB037545 Pseudomonas aeruginosa gene for 16S ribosomal RNA,
strain:ATCC 27853.
2. Nucleotide (GenBank) : AF094719 Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA
gene, partial sequence.
3. Nucleotide (GenBank) : M63957 Pseudomonas aeruginosa heat shock protein (groES and groEL)
genes, complete cds.
4. Nucleotide (GenBank) : U07832 Pseudomonas aeruginosa helicase I (helA) gene, partial cds.
5. Nucleotide (GenBank) : U64101 Pseudomonas aeruginosa heat shock protein (groesEL) gene, partial
cds.
6. Nucleotide (GenBank) : U67855 Pseudomonas aeruginosa UDP3OacylGlcNAc
deacetylase (lpxC)
gene, complete cds.
7. Nucleotide (GenBank) : X65933 P. aeruginosa pa1L
gene for PAI
galactophilic lectin.
【Supplier来源】BioVector NTCC Inc.
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