pEXP-AD502 BioVector NTCC质粒载体菌种细胞基因保藏中心

pEXP-AD502 BioVector NTCC质粒载体菌种细胞基因保藏中心

pEXP-AD502酵母双杂交载体，带有ADH启动子和终止子，GAL4AD，Gateway技术兼容，Amp抗性。TRP1酵母筛选标记。
pEXP-AD502 is an Activation Domain (AD) Gateway® Expression Vector. This
plasmid is used to construct a cDNA or genomic library for identifying proteins
(AD-Y) that interact with the fusion protein (DB-X).

Features of this vector include:
• the sequence encoding the GAL4 Activation Domain (amino acid 768-881)
fused to the nuclear localization signal from SV40 Large T antigen,
• the ARS4/CEN6 sequence for replication and low-copy-number maintenance
in yeast,
• the TRP1 gene for selection in yeast on medium lacking tryptophan,
• the constitutive moderate-strength promoter and transcription terminator of
the yeast Alcohol Dehydrogenase gene (ADH1) expressing the GAL4 AD,
• a pUC-based replication origin and ampicillin resistance gene (Apr
) for
replication and maintenance in E. coli, and
• two recombination sites, attB1 and attB2, flanking a multiple cloning site,
including Sal I and Not I sites for generation of cDNA libraries using the
SuperScript™ Plasmid System with Gateway® Technology for cDNA
Synthesis and Plasmid Cloning.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net