BxPC-3 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BxPC-3 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
BxPC-3 cell line细胞株
BxPC-3 cell line
Cat No.: NTCC691352

Organism Homo sapiens, human
Tissue pancreas
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 61 years
Gender Female
Applications This cell line is a suitable transfection host.
Images  

Clinical Data female
Genes Expressed mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)
Cellular Products mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)
Tumorigenic Yes
Effects Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments The cells do not express the cystic fibrosis transmembrane conductance regulator (CFTR).
Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks are recommended for subculturing this product.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions Temperature: 37°C
STR Profile
D5S818: 11
D13S317: 11
D7S820: 10,13
D16S539: 9,11
vWA: 14,18
THO1: 9
Amelogenin: X
TPOX: 8
CSF1PO: 13
Deposited As Homo sapiens
References Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

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