pCAMBIA1300-GFP植物绿色荧光表达载体可体外转录-BioVector NTCC保藏中心

LOCUS       AF234296                8958 bp    DNA     circular SYN 24-APR-2000
DEFINITION  Binary vector pCAMBIA-1300, complete sequence.
ACCESSION   AF234296
VERSION     AF234296.1  GI:7638064
KEYWORDS    .
SOURCE      Binary vector pCAMBIA-1300
  ORGANISM  Binary vector pCAMBIA-1300
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
            for plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
   PUBMED   7919218
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     A comprehensive set of modular vectors for advanced manipulations
            and efficient transformation of plants
  JOURNAL   Unpublished
  REMARK    Full description of constructs
REFERENCE   3  (bases 1 to 8958)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
            GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Binary vector pCAMBIA-1300"
                     /mol_type="other DNA"
                     /db_xref="taxon:118388"
     CDS             join(8944..8958,1..219)
                     /codon_start=1
                     /product="LacZ alpha fragment"
                     /protein_id="AAF65336.1"
                     /db_xref="GI:7638065"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    28..78
                     /note="pUC18 MCS; polylinker"
     misc_feature    298..323
                     /note="right border T-DNA repeat"
     misc_feature    complement(1364..2364)
                     /note="STA region from pVS1 plasmid"
     rep_origin      complement(2957..3957)
                     /note="pVS1-REP; replication origin from pVS1"
     misc_feature    complement(4367..4627)
                     /note="bom site from pBR322"
     rep_origin      complement(4767..5047)
                     /note="pBR322 origin of replication"
     CDS             complement(5338..6132)
                     /note="aadA (kanamycin resistance) gene amplified from
                     pIG121Hm"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAF65338.1"
                     /db_xref="GI:7638067"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6557..6582
                     /note="left border repeat from C58 T-DNA"
     misc_feature    6649..6857
                     /note="CaMV 3'UTR (polyA signal)"
     CDS             complement(6873..7898)
                     /note="hptII (hygromycin resistance) gene"
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /protein_id="AAF65337.1"
                     /db_xref="GI:7638066"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKK"
     promoter        complement(7934..8714)
                     /note="CaMV35S; 35S promoter from CaMV"
     promoter        8862..8916
                     /note="PlacZ; lacZ promoter"
ORIGIN      
        1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
       61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      181 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
      241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
      301 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
      361 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      421 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
      481 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
      541 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
      601 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
      661 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
      721 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
      781 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
      841 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
      901 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
      961 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     1021 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     1081 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     1141 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     1201 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     1261 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     1321 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     1381 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
     1441 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     1501 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     1561 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     1621 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     1681 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     1741 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     1801 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     1861 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     1921 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     1981 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     2041 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     2101 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     2161 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     2221 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     2281 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     2341 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     2401 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     2461 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     2521 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     2581 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     2641 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
     2701 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2761 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2821 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2881 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2941 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     3001 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     3061 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     3121 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     3181 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     3241 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     3301 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     3361 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     3421 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     3481 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3541 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3601 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3661 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3721 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3781 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3841 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3901 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3961 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     4021 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     4081 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     4141 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     4201 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4261 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4321 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     4381 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4441 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     4501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5101 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta
     5341 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
     5401 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
     5461 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
     5521 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
     5581 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
     5641 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
     5701 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
     5761 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
     5821 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
     5881 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
     5941 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
     6001 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
     6061 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
     6121 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
     6181 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
     6241 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
     6301 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     6361 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     6421 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     6481 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     6541 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     6601 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
     6661 tctggatttt agtactggat tttggtttta ggaattagaa attttattga tagaagtatt
     6721 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
     6781 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
     6841 ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg
     6901 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     6961 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     7021 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     7081 tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga
     7141 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     7201 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     7261 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     7321 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     7381 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     7441 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     7501 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt
     7561 tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     7621 cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc
     7681 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     7741 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     7801 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     7861 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct
     7921 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     7981 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
     8041 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     8101 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     8161 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     8221 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     8281 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     8341 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
     8401 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     8461 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     8521 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     8581 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     8641 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     8701 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
     8761 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8821 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8881 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8941 gctatgacca tgattacg
//