Laconic/pcDNA3.1(-)

Laconic/pcDNA3.1(-)
启动子: CMV,T7，SV40
复制子: pUC ori,f1 ori,SV40 ori
终止子: SV40 poly(A) signal,bGH poly(A) signal
质粒分类: 哺乳细胞，乳酸生物传感器
质粒大小: 7648bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物: BGH:TAGAAGGCACAGTCGAGG
质粒简介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,

质粒图谱

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