pGL4.23 [luc2/minP]

pGL4.23 [luc2/minP]
复制子: pUC ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞，信号通路报告载体
质粒大小: 4283bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导，瞬时表达
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物
质粒简介
The pGL4.23[luc2/minP] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4.23[luc2/minP] Vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and a luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli.

质粒图谱

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