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pCDNA3.1 EYFP H148Q/I152L
Vector backbone pcDNA3.1
(Search Vector Database)
Backbone manufacturer Invitrogen
Backbone size w/o insert (bp) 5400
Vector type Mammalian Expression
Selectable markers Neomycin (select with G418)
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT
Gene/Insert name EYFP H148Q/I152L
Species A. victoria
Insert Size (bp) 700
Mutation EYFP from pEYFP (Clontech) with H148Q and I152L mutations
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site HindIII (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer CMV-F
3′ sequencing primer BGHrev
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
Vector backbone pcDNA3.1
(Search Vector Database)
Backbone manufacturer Invitrogen
Backbone size w/o insert (bp) 5400
Vector type Mammalian Expression
Selectable markers Neomycin (select with G418)
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT
Gene/Insert name EYFP H148Q/I152L
Species A. victoria
Insert Size (bp) 700
Mutation EYFP from pEYFP (Clontech) with H148Q and I152L mutations
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site HindIII (not destroyed)
3′ cloning site EcoRI (not destroyed)
5′ sequencing primer CMV-F
3′ sequencing primer BGHrev
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
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