PG13-luc (wt p53 binding sites)

PG13-luc (wt p53 binding sites)
Luciferase reporter containing 13 copies of the p53-binding consensus sequence
PG13-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.

PG13 contains 13 copies of the p53-binding consensus sequence.

Direct repeats of the oligonucleotide PG (5'-CCAGGCAAGTCCAGGCAGG-3') were used for the
p53 binding sequences.

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