pcDNA3 GFP LIC cloning vector (6D)哺乳绿色荧光表达质粒
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- 货 号：pcDNA3 GFP LIC cloning vector (6D)哺乳绿色荧光表达质粒
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pcDNA3 GFP LIC cloning vector (6D)哺乳绿色荧光表达质粒
This is an empty LIC vector derived from pcDNA3. It adds a GFP gene to the C-terminus of your protein of interest.
GFP has a excitation max of 489 nm and an emission max of 510 nm.
To clone into this vector, add the following tags to your PCR primers:
LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)
LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC
Do NOT include a stop codon with your reverse primer.
T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
This is an empty LIC vector derived from pcDNA3. It adds a GFP gene to the C-terminus of your protein of interest.
GFP has a excitation max of 489 nm and an emission max of 510 nm.
To clone into this vector, add the following tags to your PCR primers:
LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)
LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC
Do NOT include a stop codon with your reverse primer.
T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
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