pME6032广宿主载体含有Tac强启动子-BioVector NTCC保藏中心提供

pME6032

特点：

广宿主载体

含有Tac强启动子

图谱Map：

序列Sequence

Shuttle vector pME6032, complete sequence

GenBank: DQ645594.1

FASTA Graphics

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Features

Sequence

LOCUS       DQ645594                9816 bp    DNA    circular SYN 27-JUN-2006

DEFINITION Shuttle vector pME6032, complete sequence.

ACCESSION   DQ645594

VERSION     DQ645594.1 GI:109452800

KEYWORDS   .

SOURCE     Shuttle vector pME6032

ORGANISM  Shuttle vector pME6032

          other sequences; artificial sequences; vectors.

REFERENCE  1  (bases 1 to 9816)

AUTHORS   Heeb,S., Itoh,Y., Nishijyo,T.,Schnider,U., Keel,C., Wade,J.,

          Walsh,U., O'Gara,F. and Haas,D.

TITLE     Small, stable shuttlevectors based on the minimal pVS1 replicon

          for use in gram-negative, plant-associated bacteria

JOURNAL   Mol. Plant MicrobeInteract. 13 (2), 232-237 (2000)

 PUBMED   10659714

REFERENCE  2  (bases 1 to 9816)

AUTHORS   Heeb,S., Blumer,C. andHaas,D.

TITLE     Regulatory RNA asmediator in GacA/RsmA-dependent global control of

          exoproduct formation in Pseudomonas fluorescens CHA0

JOURNAL   J. Bacteriol. 184 (4),1046-1056 (2002)

 PUBMED   11807065

REFERENCE  3  (bases 1 to 9816)

AUTHORS   Heeb,S.

TITLE     Direct Submission

JOURNAL   Submitted (19-MAY-2006)Institute of Infection, Immunity and

          Inflammation, University of Nottingham, Centre for Biomolecular

          Sciences, NottinghamNG7 2RD, United  Kingdom

FEATURES             Location/Qualifiers

   source          1..9816

                    /organism="Shuttlevector pME6032"

                    /mol_type="otherDNA"

                   /db_xref="taxon:390901"

   CDS             195..881

                    /note="ORF1; notrequired for stable maintenance"

                    /codon_start=1

                    /transl_table=11

                    /product="pVS1resolvase"

/protein_id="ABG33931.1"

                   /db_xref="GI:109452801"

                   /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG

                    TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA

                   DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR

                   ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA

                    AENERQEEQA"

CDS             878..1093

                    /note="ORF2; notrequired for stable maintenance"

                    /codon_start=1

                    /transl_table=11

                   /product="hypothetical protein"

/protein_id="ABG33932.1"

                   /db_xref="GI:109452802"

                   /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEIL

                   AGLSDAKLAAWPASLAAEETERRRLKR"

    CDS             1180..1809

                    /note="pVS1partitioning protein"

/codon_start=1

                    /transl_table=11

                    /product="StaA"

/protein_id="ABG33933.1"

                   /db_xref="GI:109452803"

                   /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA

                   RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL

                   IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE

                   LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

CDS             1830..2045

                    /note="pVS1partitioning protein"

                    /codon_start=1

                    /transl_table=11

                    /product="ParG"

/protein_id="ABG33934.1"

                    /db_xref="GI:109452804"

                   /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDH

                   TKLKMYAVRQGKTITELLSEYIAQLPE"

    CDS             2238..3311

                    /note="pVS1replication protein"

/codon_start=1

                    /transl_table=11

                    /product="RepA"

/protein_id="ABG33935.1"

                   /db_xref="GI:109452805"

                   /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES

                   WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR

                   LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG

                   DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV

                   SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG

                   RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA

                   GQAGPILVMRYRNLIEGEASAGS"

    rep_origin      3377..3571

                    /note="pVS1 origin ofreplication"

rep_origin      4264..5089

                    /note="p15A origin ofreplication"

   oriT            5068..5089

                    /note="p15A origin oftransfer"

   terminator      5148..5414

                    /note="T4transcription terminator"

   misc_feature    5402..5421

                    /note="sequencingoligonucleotide P6032:

                   5'-CCCTCACTGATCCGCTAGTC-3'"

   misc_feature    5416..5473

                    /note="multiplecloning site"

   misc_feature   complement(5468..5542)

                    /note="tac promoter,5'UTR and RBS followed by EcoRI"

   RBS            complement(5478..5481)

   misc_feature   complement(5508..5527)

                    /note="sequencingoligonucleotide PTAC:

                   5'-CGGCTCGTATAATGTGTGGA-3'"

   misc_feature    complement(5509)

                    /note="start oftranscription"

   -10_signal     complement(5514..5520)

   -35_signal      complement(5537..5542)

   CDS             5977..6792

                    /note="tac promoterrepressor"

                    /codon_start=1

                    /transl_table=11

                    /product="LacIQrepressor"

/protein_id="ABG33936.1"

                   /db_xref="GI:109452806"

                   /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS

                   RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV

                    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW

                   HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE

                   SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"

CDS             complement(7821..8498)

                    /note="RK2tetracycline resistance repressor protein"

                    /codon_start=1

                    /transl_table=11

                    /product="TetR"

                   /protein_id="ABG33937.1"

                   /db_xref="GI:109452807"

                   /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLG

                   VQQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAY

                   RDGARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAG

                    DSDAGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVE

                    GPRKGDD"

   CDS             8577..9776

                    /note="RK2tetracycline resistance protein"

                    /codon_start=1

                    /transl_table=11

                    /product="TetA"

                   /protein_id="ABG33938.1"

                   /db_xref="GI:109452808"

                   /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH

                    YGILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIG

                   RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH

                   APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV

                   FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE

                   RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG

                   QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG

                    AGQRADR"

ORIGIN    

      1 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt

     61 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt

    121 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc

    181 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca

    241 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca

    301 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc

    361 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca

    421 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta

    481 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt

    541 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg

    601 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga

    661 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac

    721 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc

    781 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc

    841 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca

    901 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt

    961 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc

   1021 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg

   1081 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat

   1141 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt

   1201 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa

   1261 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg

   1321 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac

   1381 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg

   1441 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca

   1501 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag

   1561 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc

   1621 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt

   1681 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca

   1741 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa

   1801 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg

   1861 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag

   1921 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg

   1981 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag

    2041agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg

   2101 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc

   2161 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc

   2221 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg

   2281 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca

   2341 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag

   2401 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg

   2461 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca

   2521 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc

   2581 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg

   2641 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat

   2701 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac

   2761 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca

   2821 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc

   2881 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa

   2941 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag

   3001 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca

   3061 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt

   3121 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca

   3181 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg

   3241 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg

   3301 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc

   3361 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga

   3421 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag

   3481 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta

   3541 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc

   3601 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc

    3661 ctatcgcggc cgctggccgc tcaaaaatggctggcctacg gccaggcaat ctaccagggc

   3721 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag

   3781 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa

   3841 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca

   3901 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc

   3961 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt

   4021 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc

   4081 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat

   4141 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt

   4201 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac

   4261 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa

   4321 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg

   4381 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct

   4441 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag

   4501 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca

   4561 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc

   4621 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg

   4681 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt

   4741 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct

   4801 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt

   4861 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg

   4921 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta

   4981 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca

   5041 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc

   5101 tgcctcgtga agaaggtgtt gctgactcat accaggctag tccgaggagc tttatgcttg

   5161 taaaccgttt tgtgaaaaaa tttttaaaat aaaaaagggg acctctaggg tccccaatta

   5221 attagtaata taatctatta aaggtcattc aaaaggtcat ccaccggatc aattcccctg

   5281 ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt

   5341 gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa

   5401 accctcactg atccgctagt ccgaggcctc gagatctatc gatgcatgcc atggtacccg

   5461 ggagctcgaa ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata

   5521 cgagccgatg attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg

   5581 tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt

   5641 gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt

   5701 ggtgcaccgt gcagtcgata agcccggatc agcttgcaat tcgcgcgcga aggcgaagcg

   5761 gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag

   5821 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc

    5881gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac

   5941 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc

   6001 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc

   6061 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa

   6121 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg

   6181 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat

   6241 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat

   6301 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg

   6361 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta

   6421 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa

   6481 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc

   6541 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg

   6601 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg

   6661 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc

   6721 aaacaggatt ttcgcctgct ggggcaaacc acgtggaccg cttgctgcaa ctctctcagg

   6781 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc

   6841 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg

   6901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag

   6961 cgcgaattgc aagctggccg acgcgctggg ctacgtcttg ctggcgttcg gatccgatat

   7021 cgccgtggcg gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac

   7081 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg

   7141 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc

   7201 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat

   7261 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac

   7321 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt

   7381 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa

   7441 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc

    7501 cctcactttc tggctggatg atggggcgattcaggcctgg tatgagtcag caacaccttc

   7561 ttcacgaggc agacctcagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc

   7621 ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg

   7681 tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct

   7741 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg

   7801 cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca

   7861 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg

   7921 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc

   7981 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg

   8041 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa

   8101 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt

   8161 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg

   8221 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta

   8281 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag

   8341 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga

   8401 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc

   8461 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag

   8521 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga

   8581 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg

   8641 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca

   8701 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg

   8761 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg

   8821 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg

   8881 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg

   8941 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt

   9001 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc

   9061 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc

   9121 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt

   9181 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca

   9241 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc

   9301 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg

   9361 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc

   9421 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga

   9481 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag

   9541 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg

   9601 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt

   9661 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc

    9721tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt

   9781 ggaaacgata ggccgctcta gaactagcta gaacta

//

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