pCDNA3.1-3×HA-N细胞表达质粒载体

pCDNA3.1-3×HA-N细胞表达质粒载体

pCDNA3.1-3×HA-N细胞过表达载体，MCS区目的基因N端带有3xHA标签，Amp抗性，G418细胞筛选标记。
启动子: CMV promoter
复制子: pUC ori，F1 ori
终止子: BGH poly（A）signal
质粒分类: 哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒
质粒大小: 5541bp
质粒标签: N-3×HA
原核抗性: 氨苄青霉素Amp（100μg/ml）
筛选标记: 新霉素Neo/G418
克隆菌株: DH5α等大肠杆菌
培养条件: 37℃，有氧，LB
表达宿主: 293T等哺乳细胞
培养条件: 37℃，5%CO2
诱导方式: 无需诱导
5'测序引物: pCDNA3.1-F（CTAGAGAACCCACTGCTTAC）
3'测序引物: pCDNA3.1-R（TAGAAGGCACAGTCGAGG）
质粒简介
      pCDNA3.1-3×HA-N质粒是一种高效表达的哺乳质粒。该质粒含有Amp抗性，可转至DH5α中转化。培养条件为LB培养基，37℃。
      pCDNA3.1-3×HA-N vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site.High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Neomycin resistance gene for selection of stable cell lines. There are three HA tags on the N side.Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
质粒图谱

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