首页 » p426-SNR52p-gRNA.CAN1.Y-SUP4t plasmid酵母基因编辑质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

p426-SNR52p-gRNA.CAN1.Y-SUP4t plasmid酵母基因编辑质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

  • 价　　格：¥39865
  • 货　　号：p426-SNR52p-gRNA.CAN1.Y-SUP4t
  • 产　　地：北京
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p426-SNR52p-gRNA.CAN1.Y-SUP4t


Yeast Expression, CRISPRChurchCAN1.y gRNA (Saccharomyces cerevisiae)Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res

  • PURPOSE
    Encodes a gRNA that targets Can1.Y in yeast.
gRNA target sequence GATACGTTCTCTATGGAGGA


BACKBONE

  • Vector backbone
    p426

  • Backbone size w/o insert (bp)5886

  • Total vector size (bp)6274

  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

GENE/INSERT

  • Gene/Insert name
    CAN1.y gRNA
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    488
  • Entrez Gene
    CAN1(a.k.a. YEL063C)

  • PromoterSNR52

CLONING INFORMATION

  • Cloning methodUnknown

  • 5′ sequencing primerT3 promoter

  • 3′ sequencing primerT7 promoter


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