MOLM-13 cells BioVector NTCC质粒载体菌种细胞基因保藏中心

MOLM-13 cells BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:MOLM-13 DSMZ no.: ACC 554 Species:human (Homo sapiens) Cell type:acute myeloid leukemia Origin: established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 Reference(s): 14869, 14743, 14493, 15073, 16558 Biosafety level: 1 Permissions and restrictions: ADSMZ Cell Culture Data: Morphology: most cells are round growing in suspension Medium: 80-90% RPMI 1640 + 10-20% h.i. FBS Subculture: split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after recovery from liquid nitrogen, but cells recover after 4-5 days Incubation: at 37 °C with 5% CO2 Doubling time: ca. 50 hours Harvest: cell harvest of ca. 2.5 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology:
CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; image Fingerprint: multiplex PCR of minisatellite markers revealed a DNA profile identical to MOLM-14 Species:confirmed as human by PCR speciation and cytogenetics Cytogenetics: human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting MLL-MLLT3 (MLL-AF9) fusion Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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