CUT: Fully functional Cas9 enzymes designed to introduce a double-strand break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ); a mechanism which frequently causes insertions or deletions (InDels) in the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone than NHEJ and can be used to faithfully introduce specific genomic changes. Mammalian Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication hCas9 Cas9 Mammalian Expression, CRISPR CMV Church RNA-Guided Human Genome Engineering via Cas9.Science. 2013 Jan 3. Add to Cart JDS246 mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag Mammalian Expression, CRISPR CMV Joung Joung lab CRISPR-Cas system (unpublished) Add to Cart lentiCas9-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR EFS-NS, EFS-NS Blasticidin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Add to Cart lentiCRISPR v2 Cas9 (Synthetic), Puromycin resistance Mammalian Expression, Lentiviral, CRISPR EFS-NS, EFS-NS Puromycin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Add to Cart pCas9_GFP Cas9-2A-GFP (Synthetic) Mammalian Expression, CRISPR CAG Musunuru Cas9 GFP plamsids(unpublished) Add to Cart pCW-Cas9 humanized S. pyogenes Cas9 Mammalian Expression, Lentiviral, CRISPR Tet ON Puromycin Sabatini Genetic screens in human cells using the CRISPR-Cas9 system. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Add to Cart pL-CRISPR.EFS.GFP SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-eGFP (Synthetic) Lentiviral, CRISPR EFS, hU6 Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pLV hUbC-Cas9-T2A-GFP humanized Cas9 T2A GFP (Other) Mammalian Expression, Lentiviral, CRISPR hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Add to Cart pMJ920 Cas9 (Synthetic) Mammalian Expression, CRISPR CMV Doudna RNA-programmed genome editing in human cells. elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29. Add to Cart pSpCas9 (PX165) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9(BB)-2A-GFP (PX458) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9(BB)-2A-Puro (PX459) hSpCas9-2A-Puro (Synthetic) Mammalian Expression, CRISPR Cbh Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9(BB)-2A-Puro (PX459) V2.0 hSpCas9-2A-Puro V2.0 (Synthetic) Mammalian Expression, CRISPR Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pST1374-NLS-flag-linker-Cas9 Human codon optimized Cas9 Mammalian Expression, CRISPR CMV Blasticidin Huang Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46. Add to Cart pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Puromycin Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems.Science. 2013 Jan 3. Add to Cart pX330-U6-Chimeric_BB-CBh-hSpCas9 humanized S. pyogenes Cas9 Mammalian Expression, CRISPR CBh Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems.Science. 2013 Jan 3. Add to Cart PX551 SpCas9 (Synthetic) Mammalian Expression, AAV, CRISPR pMecp2 Zhang In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. Add to Cart pCAG-T3-hCAS-pA Codon optimized Cas9 (Synthetic) Mammalian Expression, CRISPR CAG promoter Fujii Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013 Aug 30. Add to Cart pL-CRISPR.EFS.tRFP SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-tRFP (Synthetic) Lentiviral, CRISPR Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart LSL-Cas9-Rosa26TV Cas9 (Synthetic), EGFP Mammalian Expression, Mouse Targeting, Cre/Lox, CRISPR CAG, CAG Neomycin ; DTA Zhang CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Add to Cart M-SPcas Cas9 (Other) CRISPR CMV Neomycin Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Add to Cart p3s-Cas9HC Cas9 Mammalian Expression, CRISPR CMV Kim Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507. Add to Cart pCAG-hCas9 hCas9 Mammalian Expression, CRISPR CAG Neomycin Hatada Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system. Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 10.3390/ijms141019774. Add to Cart pL-CRISPR.SFFV.GFP SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-eGFP (Synthetic) Lentiviral, CRISPR Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pLSC-5 SpCas9(574-1368), SpCas9(2-573) Mammalian Expression, Lentiviral, CRISPR EFS, EFS Puromycin Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart pSECC Cas9 (Other), Cre Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR EFS, EFS (after Cas9-2A) Jacks Rapid modelling of cooperating genetic events in cancer through somatic genome editing. Nature. 2014 Oct 22. doi: 10.1038/nature13906. Add to Cart pSQT834 Csy4-T2A-Cas9-NLS (Homo sapiens) Mammalian Expression, CRISPR CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. Add to Cart Puro-Cas9 donor hSpCas9 (Other) Mammalian Expression, CRISPR Puromycin Huangfu An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Add to Cart pX330A-1x2 humanized S. pyogenes Cas9 nuclease (Other) Mammalian Expression, CRISPR CBh Yamamoto Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Add to Cart Eef1a-1955/-1>nls::Cas9::nls nls::Cas9::nls (Other) CRISPR Ciinte.Eef1a (EF1alpha) -1955/-1 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Add to Cart lentiCas9-EGFP Cas9 (Synthetic), EGFP (Other) Mammalian Expression, Lentiviral, CRISPR EFS-NS, EFS-NS Zhang Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis(unpublished) Add to Cart Mesp-1916/-1>nls::Cas9::nls nls::Cas9::nls (Other) CRISPR Ciinte.Mesp -1916/-1 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Add to Cart pAdSh.PGK.Cas9 Human codon-optimized S. pyogenes Cas9 (Synthetic) Mammalian Expression, Adenoviral, CRISPR Neomycin Goncalves Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells.Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. Add to Cart pHL-EF1a-SphcCas9-iP-A CRISPR Cas9 (Other) Mammalian Expression, CRISPR Puromycin Hotta Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. Add to Cart piCRg Entry hSpCas9 Mammalian Expression Huangfu An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Add to Cart pL-CRISPR.EFS.PAC SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-PAC (Synthetic) Lentiviral, CRISPR Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pL-CRISPR.SFFV.PAC SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-PAC (Synthetic) Lentiviral, CRISPR Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pL-CRISPR.SFFV.tRFP SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-tRFP (Synthetic) Lentiviral, CRISPR Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pLenti-OC-IRES-BSD OCT1 (Homo sapiens), Cas9 (Other) Mammalian Expression, Lentiviral CMV Blasticidin Wei High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 2014 Apr 9. doi: 10.1038/nature13166. Add to Cart pLKO5d.EFS.SpCas9.P2A.BSD SpCas9 (Other), EFS (Homo sapiens), P2A-BSD (Synthetic) Lentiviral, CRISPR Blasticidin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pLKO5d.EFS.SpCas9.P2A.PAC SpCas9 (Other), EFS (Homo sapiens), P2A-PAC (Synthetic) Lentiviral, CRISPR Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Add to Cart pSaCas9_GFP SaCas9-2A-GFP (Synthetic) Mammalian Expression, CRISPR CAG Musunuru Staphylococcus aureus Cas9(unpublished) Add to Cart pSQT817 Cas9 Mammalian Expression, CRISPR CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. Add to Cart PX851 SpCas9 (aa 2-535) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart PX852 SpCas9 (aa536-1368) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart PX853 SpCas9 (aa 2-573) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart PX854 SpCas9 (aa573-1368) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart Sox1/2/3-2373/+3>nls::Cas9::nls nls::Cas9::nls (Other) CRISPR Ciinte.Sox1/2/3 (SoxB1) -2373/+3 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Add to Cart Plasmid Gene/Insert Vector Type Promoter PI Publication pCas9 tracr/Cas9 Bacterial Expression, CRISPR ; E.coli Marraffini RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. Add to Cart pET-28b-Cas9-His Cas9 (Other) Bacterial Expression, CRISPR Schier Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. Add to Cart pMJ806 Cas9 Bacterial Expression, CRISPR T7 Doudna A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012 Jun 28. Add to Cart pwtCas9-bacteria wild-type Cas9 Bacterial Expression, CRISPR pLtetO-1 Qi Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Add to Cart pCRISPomyces-2 sSpCas9 (Synthetic), gRNA cassette (Synthetic) Bacterial Expression, CRISPR rpsL(XC)-BbsI, gapdhp(EL) Zhao High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol. 2014 Dec 8. Add to Cart pET28a/Cas9-Cys Cas9-Cys (Other) Bacterial Expression T7 Promoter Kim Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res. 2014 Apr 2. Add to Cart DS-SPcas Cas9 (Other), tracrRNA precursor (Other) CRISPR proC, tracrRNA promoter Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Add to Cart pCas cas9 (Other) Bacterial Expression, CRISPR ; Lambda Red recombinase native cas9 promoter Yang Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. Add to Cart pCRISPomyces-1 sSpCas9 (Synthetic), tracrRNA (Other), crRNA cassette (Synthetic) Bacterial Expression, CRISPR rpsLp(XC)-BbsI, rpsLp(CF), gapdhp(EL) Zhao High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol. 2014 Dec 8. Add to Cart pET-Cas9-6xHis Cas9_6xHis (Other) Bacterial Expression T7 Liu Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. Add to Cart pET-Cas9-NLS-6xHis Cas9-NLS-6xHis (Other) Bacterial Expression T7 Liu Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. Add to Cart pET-NLS-Cas9-6xHis NLS-Cas9-6xHis (Other) Bacterial Expression T7 Liu Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. Add to Cart pTrex-b-NLS-hSpCas9 NLS-hSpCas9 CRISPR ; Trypanosoma cruzi expression Ribo-HX1 Tarleton CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi. MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14. Add to Cart SP-Cas9 SP-CAS9 (Other) Bacterial Expression, CRISPR T7 Geijsen Efficient Intracellular Delivery of Native Proteins. Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028. Add to Cart Drosophila Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pAc-sgRNA-Cas9 Cas9 (Synthetic), dU6-sgRNA (Drosophila melanogaster) Insect Expression, CRISPR Actin-5c, Drosophila U6 Puromycin Liu Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120. Add to Cart pBS-Hsp70-Cas9 codon optimized Cas9 Insect Expression, CRISPR Hsp70 O'Connor-Giles FlyCRISPR (unpublished) Add to Cart pHsp70-Cas9 Codon optimized Cas9 CRISPR O'Connor-Giles Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.Genetics. 2013 May 24. Add to Cart pDCC6 Cas9 (Synthetic), U6-2>sgRNA (Synthetic) CRISPR hsp70Bb, U6-96Ab Duchek Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila. G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 10.1534/g3.114.014126. Add to Cart pAct-Cas9 cas9 Insect Expression act5C Bullock Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. Add to Cart pnos-Cas9-nos cas9 Insect Expression nanos Bullock Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. Add to Cart pRB14 S. pyogenes cas9 with humanized codon bias (Other) Insect Expression tubulin Foerstemann Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.Nucleic Acids Res. 2014 Apr 19. Add to Cart Showing 1 to 10 of 10 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pDD162 (Peft-3::Cas9 + Empty sgRNA) Cas9 (Synthetic), Empty sgRNA (Other) Worm Expression, CRISPR eft-3, U6 Goldstein Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. Add to Cart Peft-3::cas9-SV40_NLS::tbb-2 3'UTR codon optimized Cas9_SV40 NLS with intron (Synthetic) CRISPR eft-3 Calarco Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. Add to Cart pMB66 Cas9 (Synthetic) Worm Expression, CRISPR hsp-16.48 Boxem CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.Genetics. 2013 Aug 26. Add to Cart peft-3::cas-9::tbb-2 3'UTR cas9 CRISPR eft-3 de Bono Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination.Nucleic Acids Res. 2013 Sep 5. Add to Cart pIK86 Cas9 (Synthetic) CRISPR, Unspecified T7 Katic Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans.Genetics. 2013 Aug 26. Add to Cart pJW1259 Deletion Worm Expression, CRISPR Ward Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.Genetics. 2014 Dec 9. pii: genetics.114.172361. Add to Cart pMB62 Cas9 (Synthetic) Worm Expression, CRISPR eft-3 Boxem CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.Genetics. 2013 Aug 26. Add to Cart pMB63 Cas9 (Synthetic) Worm Expression, CRISPR eft-3 Boxem CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.Genetics. 2013 Aug 26. Add to Cart pMB67 Cas9 (Synthetic) Worm Expression, CRISPR hsp-16.48 Boxem CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.Genetics. 2013 Aug 26. Add to Cart SP6-hCas9-Ce-mRNA hCas9 with C. elegans 5' and 3' UTRs (Homo sapiens) CRISPR SP6 Sternberg Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.Genetics. 2013 Aug 26. Add to Cart Plant Showing 1 to 23 of 23 entries Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pK7WGF2::hCas9 hCas9 (Synthetic) CRISPR ; Plant expression 35S Kanamycin Kamoun Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655. Add to Cart pFGC-pcoCas9 35SPPDK:pcoCas9:NOS cassette (Synthetic) CRISPR ; Plant expression constitutive 35SPPDK promoter Basta Sheen Sheen lab CRISPR plasmids (unpublished) Add to Cart pRGEB31 CRISPR ; plant expression Rice snoRNA U3 and dual 35S promoter Hygromycin Yang RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. Add to Cart HBT-pcoCas9 pcoCas9 (Synthetic) CRISPR ; plant expression hybrid constitutive promoter 35SPPDK Sheen Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. Add to Cart pICH41308::hCas9 hCas9 (Synthetic) CRISPR Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Add to Cart pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST 35Sp::hCas9 (Synthetic) CRISPR Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Add to Cart pRGE31 Plant Expression, CRISPR Rice snoRNA U3 and dual 35S promoter Yang RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. Add to Cart Cas9 MDC123 Glycine Max codon optimized Cas9 (Other) Plant Expression, CRISPR 2X35S Basta Stupar Codon optimized Glycine max CRISPR/Cas9(unpublished) Add to Cart p201B Cas9 Cas9 (Synthetic), BAR (Other) CRISPR 2x35S, 2x35S Basta Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Add to Cart p201G Cas9 Cas9 (Synthetic), sGFP (Synthetic) CRISPR 2x35S, 2x35S GFP Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Add to Cart p201H Cas9 Cas9 (Synthetic), hph (Other) CRISPR 2x35S, StUbi-3P Hygromycin Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Add to Cart p201N Cas9 Cas9 (Synthetic), nptII (Other) CRISPR 2x35S, StUbi-3P G418, kanamycin Parrott Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16 Add to Cart pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1 sgRNA_PDS2-Cas9-sgRNA_PDS1 (Synthetic) Plant Expression, CRISPR Kamoun Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39. Add to Cart pBUE411 gRNA scaffold (Synthetic), zCas9 (Other) Plant Expression ; plant binary vector OsU3, Ubi Basta Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pBUN411 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) CRISPR ; Plant expression Ubi1p, OsU3p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pBUN421 gRNA scaffold (Synthetic), zCas9 (Other) Plant Expression ; plant binary vector TaU3, Ubi Basta Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pHSE401 gRNA scaffold (Synthetic), zCas9 (Other) Plant Expression ; plant binary vector AtU6-26p, 35S Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pHSN401 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) CRISPR ; Plant expression 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pHUE411 gRNA scaffold (Synthetic), zCas9 (Other) Plant Expression ; plant binary vector OsU3, Ubi Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pJIT163-2NLSCas9 Cas9 (Other) Plant expression 2x35S Gao Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. Add to Cart pKSE401 gRNA scaffold (Synthetic), zCas9 (Other) Plant Expression ; plant binary vector AtU6-26p, 35S Neomycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pRGE32 Plant Expression, CRISPR Rice snoRNA U3 promoter for gRNA/PTG expression and UBI promoter for Cas9 expression Yang Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. Add to Cart pRGEB32 Plant Expression, CRISPR Rice snoRNA U3 promoter for gRNA/PTG expression and UBI promoter for Cas9 expression Hygromycin Yang Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. Add to Cart Yeast Showing 1 to 8 of 8 entries
records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication p414-TEF1p-Cas9-CYC1t Human Optimized S. pyogenes Cas9 (Homo sapiens) Yeast Expression, CRISPR TEF1 promoter TRP1 Church Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.Nucleic Acids Res Add to Cart p415-GalL-Cas9-CYC1t Human Optimized S. pyogenes Cas9 (Homo sapiens) Yeast Expression, CRISPR GalL LEU2 Church Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.Nucleic Acids Res Add to Cart pACT2-CAS9c Cas9c (Synthetic) Bacterial Expression, Yeast Expression, CRISPR yeast ADH1 promoter LEU2 Zhao Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Add to Cart pCRCT iCas9 (Other), tracrRNA (Other) Yeast Expression, CRISPR TEF1p, RPR1p URA3 Zhao Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. ACS Synth Biol. 2014 Sep 19. Add to Cart pMZ374 Cas9 (Other), rrk1:sgRNA (Other) Yeast Expression, CRISPR adh1, rrk1 ura4 Zaratiegui Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Add to Cart Cas9-NAT Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette Yeast Expression, CRISPR Nourseothricin(clonNat) Jin Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. Add to Cart pCT iCas9 (Other), tracrRNA (Other) Yeast Expression, CRISPR TEF1p, RPR1p LEU2 Zhao Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. ACS Synth Biol. 2014 Sep 19. Add to Cart pMZ222 Cas9 (Other) Yeast Expression adh1 LEU2 Zaratiegui Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Add to Cart Zebrafish Showing 1 to 10 of 10 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication MLM3613 Streptococcus pyogenes Cas9 CRISPR ; zebrafish expression CMV Joung Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. Add to Cart pCS2-nCas9n nls-zcas9-nls (Synthetic) Mammalian Expression, CRISPR CMV Chen Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.Proc Natl Acad Sci U S A. 2013 Aug 5. Add to Cart pST1374-NLS-flag-linker-Cas9 Human codon optimized Cas9 Mammalian Expression, CRISPR CMV Blasticidin Huang Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46. Add to Cart pT3TS-nCas9n Cas9 (Synthetic) Bacterial Expression, CRISPR T3 Chen Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.Proc Natl Acad Sci U S A. 2013 Aug 5. Add to Cart MLM3639 Streptococcus pyogenes Cas9-3X Flag CRISPR ; zebrafish expression CMV Joung Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. Add to Cart pCS2+hSpCas9 humanized S.pyogenes Cas9 Mammalian Expression, CRISPR ; medaka, zebrafish SP6 Kinoshita Kinoshita lab Cas9 plasmids(unpublished) Add to Cart pCS2-Cas9 Cas9 (Other) Mammalian Expression, CRISPR ; xenopus and zebrafish expression SP6 Schier Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. Add to Cart pME-Cas9 Cas9 (Danio rerio) CRISPR ; Tol2 Middle Entry vector for Gateway Zon A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish. Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032. Add to Cart pME-Cas9-T2A-GFP Cas9-T2A-GFP (Danio rerio) CRISPR ; Tol2 Middle Entry vector for Gateway Zon A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish. Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032. Add to Cart pME-Cas9 codon optimized Cas9 (Other) CRISPR Chen Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics. 2015 Apr 8. pii: genetics.115.176917. Add to Cart Xenopus Showing 1 to 1 of 1 entries Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pCS2-3xFLAG-NLS-SpCas9-NLS 3xFLAG-NLS-SpCas9-NLS CRISPR ; Xenopus tropicalis expression CMV Chen Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.Development. 2014 Jan 8. Add to Cart Interfere A catalytically inactive Cas9 (dCas9) or dCas9-repressor peptide fusion can be used to knock-down gene expression by interfering with transcription of the gene. Design your gRNA sequence to direct the dCas9 repressor to a specific genomic sequence. Potential target locations can include promoter regions, regulatory regions, and early coding regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9 repressor. Mammalian Cas9 Plasmid Repressor Promoter Validated in Delivery Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) Cas9m2 dCas9 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church Cas9m3 dCas9 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church Cas9m4 dCas9 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church pcDNA-dCas9 dCas9 CMV 293T Transfection Neo/G418 pSPgRNA Gersbach pLV hUbC-dCas9-T2A-GFP dCas9 hUbC Lentiviral EGFP See lab page Gersbach pAC84-pCR8-dCas9 dCas9 no promoter Gateway donor plasmid Jaenisch pSLQ1658-dCas9-EGFP dCas9 LTR HEK293T, UMUC3, HeLa, hRPE Retroviral Puromycin need separate gRNA plasmid Qi and Huang + EGFP pdCas9-humanized dCas9 MSCV LTR 293T Retroviral Puromycin pgRNA-humanized Qi pHR-SFFV-dCas9-BFP dCas9 SFFV 293T Lentiviral BFP pgRNA-humanized Qi and Weissman pHR-SFFV-dCas9-BFP-KRAB dCas9-KRAB SFFV 293T Lentiviral BFP pgRNA-humanized Qi and Weissman pHAGE TRE dCas9-KRAB dCas9-KRAB TRE hPSC Lentiviral Neo/G418 pLKO.1-puro U6 sgRNA BfuAI stuffer Wolfe and Maehr pHAGE EF1α dCas9-KRAB dCas9-KRAB EF1alpha hPSC Lentiviral Puromycin pLKO.1-puro U6 sgRNA BfuAI stuffer Wolfe and Maehr pHAGE TRE dCas9 dCas9 TRE hPSC Lentiviral Neo/G418 pLKO.1-puro U6 sgRNA BfuAI stuffer Wolfe and Maehr Bacteria Cas9 Plasmid Repressor Promoter Validated in Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) DS-SPcasN- dCas9 proC E. coli Spectinomycin gRNA_Cloning Vector Church pdCas9 dCas9 Tet E. coli, Chloramphenicol Contained on Marraffini S. pneumoniae DB17 same plasmid pdCas9-bacteria dCas9 Anhydrotetracycline E.coli Chloramphenicol pgRNA-bacteria Qi (aTc)-inducible Cas9 species = S. thermophilus (PAM = NNAGAA) DS-ST1casN- dCas9 proC E. coli Spectinomycin see paper Church Cas9 species = T. denticola (PAM = NAAAAC) DS-TDcasN- dCas9 proC E. coli Spectinomycin see paper Church Yeast Cas9 Plasmid Repressor Promoter Validated in Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) pTDH3-dCas9 dCas9 TDH3 S. cerevisiae LEU2 need separate gRNA plasmid Qi and Weissman pTDH3-dCas9-Mxi1 dCas9-Mxi1 TDH3 S. cerevisiae LEU2 need separate gRNA plasmid Qi and Weissman Activate A catalytically inactive Cas9 (dCas9) fused to a transcription activator peptide can increase transcription of a gene. Design your gRNA sequence to direct the dCas9-activator to a specific genomic sequence. Potential target locations can include promoter regions and regulatory regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator. Mammalian Cas9 Plasmid Activator Promoter Validated in Delivery Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) M-SPn-VP64 VP64 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church Cas9m4-VP64 VP64 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church Cas9m3-VP64 VP64 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church Cas9m2-VP64 VP64 CMV 293T Transfection Neo/G418 gRNA_Cloning Vector Church pcDNA-dCas9-VP64 VP64 CMV 293T Transfection Neo/G418 pSPgRNA Gersbach pLV hUbC-dCas9 VP64-T2A-GFP VP64 hUbC Lentiviral EGFP See lab page Gersbach pAC154-160Dual VP160 CBh 293T, HeLa, NIH3T3, Transfection Contained on Jaenisch mESC, embryos same plasmid pAC153-96Dual VP96 CBh 293T, HeLa, NIH3T3, Transfection Contained on Jaenisch mESC, embryos same plasmid pAC152-64Dual VP64 CBh 293T, HeLa, NIH3T3, Transfection Contained on Jaenisch mESC, embryos same plasmid pAC5-dual-dCas9VP48-sgTetO VP48 CBh 293T, HeLa Transfection Contained on Jaenisch same plasmid pAC2-dual-dCas9VP48-sgExpression VP48 CBh 293T, HeLa Transfection Contained on Jaenisch same plasmid pAC95-pmax-dCas9VP160-2A-neo VP160 CAG 293T, HeLa Transfection Neo/G418 pAC155-pCR8-sgExpression Jaenisch pAC94-pmax-dCas9VP160-2A-puro VP160 CAG 293T, HeLa Transfection Puromycin pAC155-pCR8-sgExpression Jaenisch CAGGS-160 VP160 CAG 293T, HeLa, NIH3T3, Transfection Transfection of PCR Jaenisch mESC, embryos product coding gRNA CAGGS-96 VP96 CAG 293T, HeLa, NIH3T3, Transfection Transfection of PCR Jaenisch mESC, embryos product coding gRNA CAGGS-64 VP64 CAG 293T, HeLa, NIH3T3, mESC, embryos Transfection Transfection of PCR Jaenisch product coding gRNA dCas9VP160 VP160 no promoter Gateway donor plasmid Jaenisch dCas9VP96 VP96 no promoter Gateway donor plasmid Jaenisch dCas9VP64 VP64 no promoter Gateway donor plasmid Jaenisch pAC1-pCR8-dCas9VP48 VP48 no promoter Gateway donor plasmid Jaenisch pMLM3705 VP64 CMV 293T Transfection pMLM3636 Joung pSL690 VP64 CMV 293T Transfection pMLM3636 Joung pCMV_dCas9_VP64 VP64 CMV 293T Transfection mKate pU6_gRNA_handle_U6t Lu CMVp-dCas9-3xNLS-VP64 (Construct 1) VP64 CMV/hUBC /td> 293T Transfection See lab page Lu pMSCV-LTR-dCas9-p65AD-BFP p65AD MSCV LTR 293T Retroviral BFP and Puromycin pgRNA-humanized Qi and Weissman pMSCV-LTR-dCas9-VP64-BFP VP64 MSCV LTR 293T Retroviral BFP and Puromycin pgRNA-humanized Qi and Weissman pHAGE EF1α dCas9-VP64 VP64 EF1alpha hPSC Lentiviral Puromycin pLKO.1-puro U6 sgRNA BfuAI stuffer Wolfe pHAGE TRE dCas9-VP64 VP64 TRE hPSC Lentiviral Neomycin pLKO.1-puro U6 sgRNA BfuAI stuffer Wolfe Cas9 species = N. meningitidis (PAM = NNNNGATT)
M-NMn-VP64 VP64 CMV 293T Transfection Neo/G418 see paper Church Cas9 species = S. thermophilus (PAM = NNAGAA)
M-ST1n-VP64 VP64 CMV 293T Transfection Neo/G418 see paper Church Bacteria Cas9 Plasmid Activator Promoter Validated in Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) pWJ68 RNAP omega subunit (ω), N-term on dCas9 Tet E. coli, S. pneumoniae Chloramphenicol Contained on Marraffini same plasmid pWJ66 RNAP omega subunit (ω), C-term on dCas9 Tet E. coli, S. pneumoniae Chloramphenicol Contained on Marraffini same plasmid Yeast Cas9 Plasmid Activator Promoter Validated in Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) pTPGI_dCas9_VP64 VP64 pTPGI S. cerevisiae TRP1 pRPR1_gRNA_handle_RPR1t Lu Purify A catalytically inactive Cas9 (dCas9) fused to an epitope tag(s) can be used to purify genomic DNA bound by the gRNA. Design your gRNA sequence to direct the dCas9 to a specific genomic sequence. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9. Mammalian Cas9 Plasmid Tag Promoter Delivery Selection Co-published Depositing lab gRNA Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) 3xFLAG-dCas9/pMXs-neo 3xFLAG LTR Retroviral Neo/G418 gRNA_Cloning Vector Fujii 3xFLAG-dCas9/pMXs-I2 3xFLAG LTR Retroviral human CD2 gRNA_Cloning Vector Fujii 3xFLAG-dCas9/pMXs-IG 3xFLAG LTR Retroviral EGFP gRNA_Cloning Vector Fujii 3xFLAG-dCas9/pMXs-puro 3xFLAG LTR Retroviral Puromycin gRNA_Cloning Vector Fujii 3xFLAG-dCas9/pCMV-7.1 3xFLAG CMV Transfection gRNA_Cloning Vector Fujii Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can be used to visualize specific genomic loci using fluorescent microscopy in living cells. Design your gRNA sequence to direct the dCas9-FP fusion to a specific genomic sequence. Potential target locations can include unique or repetitive regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-FP protein. Mammalian Cas9 Plasmid Fluorophore Promoter Validated in Delivery Selection Co-published gRNA Depositing lab Plasmid, if separate Cas9 species = S. pyogenes (PAM = NGG) pSLQ1658-dCas9-EGFP EGFP LTR HEK293T, UMUC3, HeLa, hRPE Retroviral Puromycin need separate gRNA plasmid Qi and Huang pHR-SFFV-dCas9-BFP BFP SFFV 293T Lentiviral pgRNA-humanized Qi and Weissman Screen (libraries) The CRISPR/Cas9 system has quickly become an important tool for the genome engineering community, but the technique has primarily been limited to focused gene knock-outs and direct transfection protocols. The vectors and sgRNA libraries described below expand upon the CRISPR family of plasmids by A) offering a lentivirus-based mechanism for sgRNA delivery and B) providing a means for large scale functional screens. Learn more about how to use pooled lentiviral CRISPR libraries. Feng Zhang Lab (libraries targeting human and mouse genes) The human genome-scale CRISPR-Cas9 knockout (GeCKO) v2 library consists of 122,417 unique guide sequences targeting 19,052 human genes and including 1000 control (non-targeting) sgRNAs. The murine library consists of 130,209 unique guide sequences targeting 20,661 murine genes. There are two versions of each library. The lentiCRISPR v2 still expresses both Cas9 and the sgRNA on the same plasmid but can produce 10-fold higher titers than the original lentiCRISPR backbone. The 2 vector format uses lentiGuide-Puro as a backbone and produces an even higher titer but must be used with cells that already contain Cas9. The lentiCas9-Blast vector is provided with this 2-plasmid version of the GeCKO library. Type ID Item Plasmid 52961 lentiCRISPR v2: Lentiviral expression of hSpCas9 and sgRNA (replaces Addgene plasmid 49535) Add to Cart Plasmid 52962 lentiCas9-Blast: Lentiviral expression of human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Add to Cart Plasmid 52963 lentiGuide-Puro: Lentiviral expression of S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Add to Cart Library Human GeCKO v2 Library, 1 plasmid system Order Here Library Human GeCKO v2 Library, 2 plasmid system Order Here Library Mouse GeCKO v2 Library, 1 plasmid system Order Here Library Mouse GeCKO v2 Library, 2 plasmid system Order Here David Sabatini Lab / Eric Lander Lab (targets human genes) The library described in this article consists of 73,151 sgRNA plasmids that cover a total of 7,114 human genes and include 100 non-targeting controls. The library has been separated into 6 distinct enriched sub-pools: 4 pools enriched for gene targets of known function (kinases, cell cycle proteins, nuclear proteins, and ribosomal proteins), 1 pool that targets genes of unknown function, and 1 control pool. Note that though each sub-pool is enriched for their respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool. There are two lentiviral vectors associated with this paper, one expressing Cas9 (pCW-Cas9) and the sgRNA backbone (pLX-sgRNA). Genetic Screens in Human Cells Using the CRISPR/Cas9 System. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2013 Dec 12. (PubMed). Type ID Item Plasmid 50661 pCW-Cas9: Inducible lentiviral expression of SpCas9. Add to Cart Plasmid 50662 pLX-sgRNA: Lentiviral expression of AAVS1-targeting sgRNA which can be replaced with custom sgRNA. Add to Cart Library 51043 Human Lentiviral sgRNA Library - Enriched Against Proteins of Unknown Function Add to Cart Library 51044 Human Lentiviral sgRNA Library - Enriched Against Kinases Add to Cart Library 51045 Human Lentiviral sgRNA Library - Enriched Against Ribosomal Proteins Add to Cart Library 51046 Human Lentiviral sgRNA Library - Enriched Against Cell Cycle Proteins Add to Cart Library 51047 Human Lentiviral sgRNA Library - Enriched Against Nuclear Proteins Add to Cart Library 51048 Human Lentiviral sgRNA Library - Enriched Against Control Proteins (most diverse pool) Add to Cart Kosuke Yusa Lab (targets mouse genes) The library described in this article consists of 87,897 unique gRNA plasmids, targeting 19,150 mouse protein coding regions. The library is designed for lentiviral expression in mouse cells. Genome-wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Bioetchnology. 2013. doi:10.1038/nbt.2800 (Article). Type ID Item Plasmid 50946 pKLV-U6gRNA(BbsI)-PGKpuro2ABFP : empty gRNA expression vector for lentiviral production. Add to Cart Library 50947 Genome-wide mouse lentiviral CRISPR gRNA library Add to Cart Feng Zhang Lab (targets human genes) The genome-scale CRISPR-Cas9 knockout (GeCKO) library originally described in Shalem*, Sanjana*, et al., Science 2014. The original library is discontinued and an updated version of this library is now available. Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (PubMed). Type ID Item Plasmid 49535 Lentiviral expression of hSpCas9 and sgRNA (lentiCRISPR v2 available here) Discontinued Library 51241 Human GeCKO Lentiviral sgRNA Library (Human GeCKO v2 available here) Discontinued Empty gRNA Vectors Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When using the CRISPR/Cas system, you will need to express both a Cas9 protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas9 act as an all-in-one vector, but their function (cut, nick, activate, interfere...) is limited to that of the Cas9 present on the plasmid. gRNA plasmids that do not co-express Cas9 require a separate plasmid that does so; however, these independent gRNA plasmids can be paired with a wide variety of Cas9 plasmids and therefore are not limited to a single Cas9 function. Mammalian gRNA Plasmid Promoter Cloning Delivery Selection Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG)
gRNA_Cloning Vector hU6 AfIII Transfection Bleocin none, need Cas9 plasmid Church pSPgRNA hU6 BbsI Transfection none, need Cas9 plasmid Gersbach phH1-gRNA human H1 BbsI Transfection none, need Cas9 plasmid Gersbach pmU6-gRNA mouse U6 BbsI Transfection none, need Cas9 plasmid Gersbach phU6-gRNA human U6 BbsI Transfection none, need Cas9 plasmid Gersbach ph7SK-gRNA human 7SK BbsI Transfection none, need Cas9 plasmid Gersbach NEW pGL3-U6-sgRNA-PGK-puromycin hU6 Transfection Puromycin none, need Cas9 plasmid Huang NEW pUC57-sgRNA expression vector T7 BsaI In vitro transcription none, need Cas9 plasmid Huang pAC155-pCR8-sgExpression hU6 BbsI Transfection none, need Cas9 plasmid Jaenisch pAC154-dual-dCas9VP160-sgExpression hU6 BbsI Transfection yes, activate Jaenisch pAC153-dual-dCas9VP96-sgExpression hU6 BbsI Transfection yes, activate Jaenisch pAC152-dual-dCas9VP64-sgExpression hU6 BbsI Transfection yes, activate Jaenisch pAC5-dual-dCas9VP48-sgTetO hU6 BbsI Transfection yes, activate Jaenisch pAC2-dual-dCas9VP48-sgExpression hU6 BbsI Transfection yes, activate Jaenisch MLM3636 hU6 BsmBI Transfection none, need Cas9 plasmid Joung pSQT1313 hU6 BsmBI Transfection none, need Cas9 plasmid Joung pU6_gRNA_handle_U6t U6 SacI Transfection EBFP none, need Cas9 plasmid Lu pgRNA-humanized mU6 BstXI + XhoI Lentiviral Puromycin, none, need Cas9 plasmid Qi mCherry pLX-sgRNA hU6 See paper Lentiviral Blasticidin none, need Cas9 plasmid Sabatini and Lander pLenti-sgRNA-Lib hU6 BsmBI Lentiviral ccdB none, need Cas9 plasmid Wei pLKO.1-puro U6 sgRNA BfuAI stuffer hU6 BfuAI Lentiviral Puromycin none, need Cas9 plasmid Wolfe NEW pLKO.1-puro U6 sgRNA BfuAI large stuffer hU6 BfuAI Lentiviral Puromycin none, need Cas9 plasmid Wolfe NEW pKLV-U6gRNA(BbsI)-PGKpuro2ABFP hU6 BbsI Lentiviral Puromycin, none, need Cas9 plasmid Yusa TagBFP lentiGuide-Puro hU6 BsmBI Lentiviral Puromycin none, need Cas9 plasmid Zhang lentiCRISPR v2 hU6 BsmBI Lentiviral Puromycin yes, cut Zhang PX462 (3rd Gen) hU6 BbsI Transfection Puromycin yes, nick (D10A) Zhang PX461 (3rd Gen) hU6 BbsI Transfection EGFP yes, nick (D10A) Zhang PX460 (3rd Gen) hU6 BbsI Transfection yes, nick (D10A) Zhang PX459 (3rd Gen) hU6 BbsI Transfection Puromycin yes, cut Zhang PX458 (3rd Gen) hU6 BbsI Transfection EGFP yes, cut Zhang PX335 (2nd Gen) hU6 BbsI Transfection yes, nick (D10A) Zhang PX334 (1st Gen) hU6 BbsI Transfection Puromycin yes, nick (D10A) Zhang PX330 (2nd Gen) hU6 BbsI Transfection yes, cut Zhang PX260 (1st Gen) hU6 BbsI Transfection Puromycin yes, cut Zhang Cas9 species = N. meningitidis (PAM = NNNNGATT) pSmart-Nm-sgRNA-BbsI hU6 BbsI Transfection none, need Cas9 plasmid Thomson and Sontheimer pSimpleII-U6-tracr- hU6 BsmBI Transfection yes, cut Thomson and U6-BsmBI-NLS- Sontheimer NmCas9-HA-NLS(s) Bacteria gRNA Plasmid Promoter Cloning Validated In Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG)
pCRISPR BsaI E. coli, Chloramphenicol none, need Cas9 plasmid Marraffini S. pneumoniae pCas9 BsaI E. coli, Chloramphenicol yes, cut Marraffini S. pneumoniae pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Drosophila gRNA Plasmid Promoter Cloning Delivery Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG) pCFD1-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection or in vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port pRB17 U6 blunt end cloning Injection or in vitro transcription none, need Cas9 plasmid Foerstemann pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison, Wildonger pU6-BbsI-chiRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles, Harrison, Wildonger C. elegans gRNA Plasmid Promoter Cloning Delivery Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG) sgRNA with U6 promoter cU6 HindIII Injection none, need Cas9 plasmid de Bono sgRNA with rpr-1 promoter rpr-1 EcoRI Injection none, need Cas9 plasmid de Bono pMB70 cU6 BsaI Transfection none, need Cas9 plasmid Boxem pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung SP6-sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg Plant gRNA Plasmid Promoter Cloning Validated In Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG) pICSL01009::AtU6p aU6 BsaI Arabidopsis none, need Cas9 plasmid Kamoun pFGC-pcoCas9 none AscI, PacI, SbfI Arabidopsis yes, cut Sheen pUC119-gRNA U6 PCR template Arabidopsis none, need Cas9 plasmid Sheen pRGEB31 rice snoRNA U3 BsaI Arabidopsis none, need Cas9 plasmid Yang pRGE31 rice snoRNA U3 BsaI Arabidopsis none, need Cas9 plasmid Yang Yeast gRNA Plasmid Promoter Cloning Validated In Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG) pRPR1_gRNA_ pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu handle_RPR1t Zebrafish gRNA Plasmid Promoter Cloning Delivery Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG)
pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Xenopus gRNA Plasmid Promoter Cloning Delivery Resistance Co-expressed Cas9 Depositing lab Enzyme(s) Cas9 species = S. pyogenes (PAM = NGG) NEW pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid Chen Nick A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick) at a specific location based on a co-expressed gRNA-defined target sequence, rather than a double-strand DNA break (Cut) produced by the wild type enzyme. Nicks are preferentially repaired in the cell by homology directed repair (HDR), using the intact strand as the template. HDR has high fidelity and rarely results in errors. Two adjacent, opposite strand nicks can cause a double strand break (DSB) and trigger error-prone non-homologous end joining (NHEJ) repair; however, in the presence of a repair template, the double nicks can be repaired by HDR. Double nicking greatly reduces unwanted off-target effects. Mammalian Showing 1 to 15 of 15 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication hCas9_D10A Cas9_D10A Mammalian Expression, CRISPR CMV Church RNA-Guided Human Genome Engineering via Cas9.Science. 2013 Jan 3. Add to Cart pCas9D10A_GFP Cas9D10A-2A-GFP (Synthetic) Mammalian Expression, CRISPR CAG Musunuru Cas9 GFP plamsids(unpublished) Add to Cart pSpCas9n(BB) (PX460) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9n(BB)-2A-GFP (PX461) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9n(BB)-2A-Puro (PX462) hSpCas9n-2A-Puro (Synthetic) Mammalian Expression, CRISPR Cbh Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pSpCas9n(BB)-2A-Puro (PX462) V2.0 hSpCas9n-2A-Puro (Synthetic) Mammalian Expression, CRISPR Puromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Add to Cart pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Puromycin Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems.Science. 2013 Jan 3. Add to Cart pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems.Science. 2013 Jan 3. Add to Cart ST1374-N-NLS-flag-linker-Cas9-D10A Cas9 nickase Mammalian Expression, CRISPR CMV Blasticidin Huang Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. Add to Cart pCAG-T3-hCasD10A-pA Codon-optimized Cas9 D10A (Synthetic) Mammalian Expression, CRISPR CAG promoter Fujii Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 10.1016/j.bbrc.2014.01.141. Add to Cart pNW3 Csy4-T2A-Cas9n (D10A) Mammalian Expression, CRISPR CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. Add to Cart pST1374-N-NLS-flag-linker-Cas9-H840A Cas9 nickase Mammalian Expression, CRISPR CMV Blasticidin Huang Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. Add to Cart lentiCas9n(D10A)-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR EFS-NS, EFS-NS Blasticidin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Add to Cart pCAG-hCas9D10A hCas9D10A (Other) Mammalian Expression CAG Neomycin Hatada pCAG-hCas9D10A(unpublished) Add to Cart pHL-EF1a-SphcCas9(D10A)-iP-A CRISPR Cas9 D10A (Other), puromycin resistance gene (Other) Mammalian Expression, CRISPR Puromycin Hotta Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. Add to Cart Drosophila Showing 1 to 1 of 1 entries records per page Search table: 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pDCC5 Cas9-D10A (Synthetic) Insect Expression, CRISPR hsp70Bb Duchek Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila. G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 10.1534/g3.114.014126. Add to Cart Plant Showing 1 to 2 of 2 entries records per page Search table: Previous 1 Plasmid Gene/Insert Promoter Selectable Marker PI Publication pBUN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) Ubi1p, AtU6-26p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pHSN501 zCas9D10A (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart Interfere A catalytically inactive Cas9 (dCas9) or dCas9-repressor peptide fusion can be used to knock-down gene expression by interfering with transcription of the gene. Design your gRNA sequence to direct the dCas9 repressor to a specific genomic sequence. Potential target locations can include promoter regions, regulatory regions, and early coding regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9 repressor. Mammalian Showing 1 to 23 of 23 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pcDNA-dCas9 dCas9 (Other) Mammalian Expression, CRISPR CMV Gersbach RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Add to Cart pdCas9-humanized dead Cas9 with 3X NLS (Homo sapiens) Mammalian Expression, Retroviral, CRISPR ; vector MSCV LTR promoter Puromycin Qi Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Add to Cart pdCas9::BFP-humanized dCas9 fused to BFP (Homo sapiens) Mammalian Expression, Retroviral, CRISPR MSCV LTR promoter Puromycin Qi Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Add to Cart pHAGE EF1α dCas9-KRAB dCas9 (Other) Mammalian Expression, Lentiviral, CRISPR EF1alpha Puromycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Add to Cart pHAGE TRE dCas9-KRAB dCas9 (Other) Mammalian Expression, Lentiviral, CRISPR TRE Neomycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Add to Cart pHR-SFFV-dCas9-BFP dCas9-BFP fusion (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR SFFV Weissman CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart pHR-SFFV-dCas9-BFP-KRAB dCas9-BFP-KRAB fusion (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR SFFV Weissman CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart pSLQ1658-dCas9-EGFP dCas9 fuse to EGFP (Homo sapiens) Mammalian Expression, Retroviral, CRISPR MSCV LTR promoter Puromycin Qi Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Add to Cart Cas9m4 Cas9m4 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart pHR-SFFV-KRAB-dCas9-P2A-mCherry KRAB-dCas9-P2A-mCherry fusion (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR SFFV mCherry Weissman Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9. Add to Cart Cas9m2 Cas9m2 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart pAC84-pCR8-dCas9 dCas9(D10A;H840A) (Homo sapiens) CRISPR ; Gateway Donor Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pcDNA3.1-CibN-dCas9-CibN dCas9-CibN (Arabidopsis thaliana) Mammalian Expression, CRISPR CMV Neomycin Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Add to Cart pHAGE TRE dCas9 dCas9 (Other) Mammalian Expression, Lentiviral, CRISPR TRE Neomycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Add to Cart pLV hUbC-dCas9-T2A-GFP humanized dead Cas9 T2A GFP (Other) Mammalian Expression, Lentiviral, CRISPR hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Add to Cart Cas9m3 Cas9m3 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart CRISPRi library sgRNA, puro-T2A-BFP Mammalian Expression, Lentiviral, CRISPR Puromycin Weissman Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9. Add to Cart pcDNA3.1-CibN-dCas9 CibN-dCas9 (Arabidopsis thaliana) Mammalian Expression, CRISPR CMV Neomycin Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Add to Cart pcDNA3.1-dCas9-CibN dCas9-CibN (Arabidopsis thaliana) Mammalian Expression, CRISPR CMV Neomycin Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Add to Cart pJZC73 sgRNA, COM-KRAB Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC74 sgRNA + 1x COM binding module, COM-KRAB Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC77 sgRNA, COM-KRAB Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC78 sgRNA + 1x COM binding module, COM-KRAB Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart Bacteria Showing 1 to 5 of 5 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter PI Publication pdCas9 tracrRNA (Other), dcas9 (Other), CRISPR array Bacterial Expression, CRISPR Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.Nucleic Acids Res. 2013 Jun 12. Add to Cart pdCas9-bacteria dCas9 (bacteria) (Other) Bacterial Expression, CRISPR pLtetO-1 Qi Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Add to Cart pMJ841 Cas9 (Other) Bacterial Expression, CRISPR T7 Doudna A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012 Jun 28. Add to Cart 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S dCas9 M1C D10A C80S H840A C574S (Other) Bacterial Expression Doudna Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature. 2014 Sep 28. doi: 10.1038/nature13769. Add to Cart DS-SPcasN- Cas9, nuclease-null (Other), tracrRNA precursor (Other) Bacterial Expression, CRISPR proC, tracdrRNA promoter Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Add to Cart Yeast Showing 1 to 5 of 5 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pTDH3-dCas9 dCas9 Yeast Expression, CRISPR TDH3 LEU2 Weissman CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart pTDH3-dCas9-Mxi1 dCas9-Mxi1 Yeast Expression, CRISPR TDH3 LEU2 Weissman CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart pJZC518 dCas9 (Other) Yeast Expression pTdh3 LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC572 sgRNA + 1x com RNA binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pTPGI_dCas9 Yeast-optimized dCas9 (Saccharomyces cerevisiae) Yeast Expression pTPGI TRP1 Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. Add to Cart Plant Showing 1 to 2 of 2 entries records per page Search table: Previous Next Plasmid Gene/Insert Promoter Selectable Marker PI Publication pBUN6I11 dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic) Ubi1p, OsU3p Bar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart pHSN6I01 dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic) 2×35Sp, AtU6-26p Hygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. Add to Cart Activate A catalytically inactive Cas9 (dCas9) fused to a transcription activator peptide can increase transcription of a gene. Design your gRNA sequence to direct the dCas9-activator to a specific genomic sequence. Potential target locations can include promoter regions and regulatory regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator. Mammalian Showing 1 to 57 of 57 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication Cas9m4-VP64 Cas9m4-VP64 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart dCAS9-VP64_GFP dCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic) Mammalian Expression, Lentiviral, CRISPR EF1A Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Add to Cart lenti dCAS-VP64_Blast dCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic) Mammalian Expression, Lentiviral, CRISPR EF1A Blasticidin Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Add to Cart pAC154-dual-dCas9VP160-sgExpression dCas9 (Synthetic) Mammalian Expression, CRISPR Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pcDNA-dCas9-VP64 dCas9-VP64 (Other) Mammalian Expression, CRISPR CMV Gersbach RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Add to Cart pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP Cas9 dead Mammalian Expression, Lentiviral, CRISPR Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. Add to Cart pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE dCas9 Mammalian Expression, Lentiviral, CRISPR dSV40 Promoter Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. Add to Cart pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS scFv-GCN4 Mammalian Expression, Lentiviral Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. Add to Cart pMSCV-LTR-dCas9-VP64-BFP dCas9-VP64-BFP fusion (Homo sapiens), Puromycin resistance Mammalian Expression, Retroviral, CRISPR LTR, PGK Puromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart pAC152-dual-dCas9VP64-sgExpression dCas9 (Synthetic) Mammalian Expression, CRISPR Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC93-pmax-dCas9VP160 dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens) Mammalian Expression, CRISPR CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC94-pmax-dCas9VP160-2A-puro dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro (Homo sapiens) Mammalian Expression, CRISPR CAGGS Puromycin Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pcDNA-dCas9-p300 Core S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pHAGE EF1α dCas9-VP64 dCas9 (Other) Mammalian Expression, Lentiviral, CRISPR EF1alpha Puromycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Add to Cart pHAGE TRE dCas9-VP64 dCas9 (Other) Mammalian Expression, Lentiviral, CRISPR TRE Neomycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Add to Cart pMLM3705 codon optimized dCas9-VP64 (Synthetic) Mammalian Expression, CRISPR CMV Joung CRISPR RNA-guided activation of endogenous human genes.Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Add to Cart pMSCV-LTR-dCas9-p65AD-BFP dCas9-p65AD-BFP fusion (Homo sapiens), Puromycin resistance Mammalian Expression, Retroviral, CRISPR LTR, PGK Puromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Add to Cart M-SPn-VP64 Cas9-VP64, nuclease-null (Other) CRISPR CMV Neomycin Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Add to Cart pAC149-pCR8-dCas9VP160 dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens) CRISPR ; Gateway Donor Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC153-dual-dCas9VP96-sgExpression dCas9 (Synthetic) Mammalian Expression, CRISPR Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC2-dual-dCas9VP48-sgExpression dCas9VP48 (Homo sapiens) Mammalian Expression, CRISPR Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC91-pmax-dCas9VP64 dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens) Mammalian Expression, CRISPR CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC95-pmax-dCas9VP160-2A-neo dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo (Homo sapiens) Mammalian Expression, CRISPR CAGGS Neomycin Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pcDNA-dCas9-p300 Core (D1399Y) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pLV hUbC-dCas9 VP64-T2A-GFP humanized dead Cas9 VP64 T2A GFP (Other) Mammalian Expression, Lentiviral, CRISPR hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Add to Cart SP-dCas9-VPR SP-dCas9-VPR (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Neomycin Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. Add to Cart Cas9m2-VP64 Cas9m2-VP64 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart Cas9m3-VP64 Cas9m3-VP64 (Other) CRISPR Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Add to Cart CMVp-dCas9-3xNLS-VP64 (Construct 1) dCas9 (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV/hUBC Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022. Add to Cart M_ST1n_VPR ST1-dCas9-VPR (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Neomycin Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. Add to Cart NLS-dCas9-trCIB1 dCas9-trCIB1 fusion (Synthetic) Mammalian Expression CMV Neomycin Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22. Add to Cart pAC1-pCR8-dCas9VP48 dCas9(D10A;H840A) fusion with VP48 activation domain (Synthetic) CRISPR ; Gateway Donor Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC147-pCR8-dCas9VP64 dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens) CRISPR ; Gateway Donor Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC148-pCR8-dCas9VP96 dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens) CRISPR ; Gateway Donor Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC5-dual-dCas9VP48-sgTetO dCas9VP48 and sgTetO (Synthetic) Mammalian Expression, CRISPR Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pAC92-pmax-dCas9VP96 dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens) Mammalian Expression, CRISPR CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Add to Cart pcDNA-dCas9-FLp300 S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA-dCas9-p300 Core (1396/1397 SY/WW) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA-dCas9-p300 Core (1645/1646 RR/EE) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA-dCas9-p300 Core (C1204R) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA-dCas9-p300 Core (Y1467F) S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pcDNA3.3-Nm-dCas9-p300 Core Nm-dCas9-p300 Core (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Neomycin Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers Nature Biotechnology (2015) Add to Cart pCMV_dCas9_VP64 dCas9_VP64 (human-codon-optimized) (Homo sapiens) Mammalian Expression, CRISPR, Synthetic Biology CMV Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. Add to Cart pJZC101 sgRNA, COM-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC116 sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by BFP Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC25 sgRNA + 1x MS2 binding module, MCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC32 sgRNA, MCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC33 sgRNA + 2x MS2 binding module, MCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC34 sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC39 sgRNA + 1x PP7, mCherry Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC41 sgRNA, PCP-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC48 sgRNA + 1x COM binding module, COM-VP64 Mammalian Expression, Lentiviral U6, CMV Marked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pLV hUbC-VP64 dCas9 VP64-T2A-GFP humanized VP64 dead Cas9 VP64 T2A GFP (Other) Mammalian Expression, Lentiviral, CRISPR Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Add to Cart pSL690 dCas9-VP64 (Synthetic) Mammalian Expression, CRISPR CMV Joung CRISPR RNA-guided activation of endogenous human genes.Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Add to Cart PX855 SpCas9 (aa 2-535) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart PX856 SpCas9 (aa536-1368) Mammalian Expression, CRISPR CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Add to Cart Bacteria Showing 1 to 2 of 2 entries records per page Search table: Previous Next Plasmid Gene/Insert Vector Type Promoter PI Publication pWJ66 tracrRNA (Other), dcas9-w (Other), CRISPR array Bacterial Expression, CRISPR Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Add to Cart pWJ68 tracrRNA (Other), w-dcas9 (Other), CRISPR array Bacterial Expression, CRISPR Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Add to Cart Yeast Showing 1 to 13 of 13 entries records per page Search table: Previous 1 Next Plasmid Gene/Insert Vector Type Promoter Selectable Marker PI Publication pTPGI_dCas9_VP64 dCas9_VP64 (codon-optimized for expression in S. cerevisiae) (Saccharomyces cerevisiae) Yeast Expression, CRISPR, Synthetic Biology pTPGI (galactose+aTc inducible) TRP1 Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.ACS Synth Biol. 2013 Sep 11. Add to Cart pAG414GPD-dCas9-VPR dCas9-VPR (Saccharomyces cerevisiae) Yeast Expression, CRISPR, Synthetic Biology GPD TRP1 Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. Add to Cart pJZC519 dCas9-VP64 (Other) Yeast Expression pTdh3 LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC545 sgRNA + 1x MS2 binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC548 sgRNA + 1x PP7 Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC572 sgRNA + 1x com RNA binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC583 sgRNA + 2x MS2 binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC593 sgRNA + MS2-PP7 RNA binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC595 MCP-VP64, dCas9 Yeast Expression pAdh, Tdh3 LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC603 sgRNA + 2x PP7 RNA binding module Yeast Expression SNR52 URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC620 MCP-VP64, PCP-VP64, dCas9 Yeast Expression pAdh, pAdh, pTdh3 LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC625 sgRNA +1x MS2, Pol II promoter with ribozyme cleavage Yeast Expression pAdh URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart pJZC638 MCP-VP64, dCas9 (Other) Yeast Expression pAdh, pGal10 LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Add to Cart 
