MKN-74 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MKN-74 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 MKN-74 cell line
Cat No.: NTCC596925

Cell Name MKN74
profile Human cell line derived from stomach cancer. Alias
animal human strain
genus Homo species sapiens
sex M age 37
identity available tissue stomach
case history stomach cancer metastasized to liver metastasis Yes
metastorgn liver genetics
lifespan infinite crisispdl
morphology epithelial-like charact
classify tumor originator Hojo,H.
register regulation
comment Moderately differentiated tubular adenocarcinoma. Mycoplasma eliminated cells by MC-210. dyear 1989
medium RPMI1640 medium with 10% heat inactivated fetal bovine serum. pasmethod Cells are washed once with PBS(-) and treated with 0.02% EDTA and 0.25% trypsin for 10-15 min at 37 C.
pascellno split ratio=1/10 race Japanese
co2conc 5 %
preporgn liver metastasis vrsummary tested
vrsdetect CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] tissueform


Reference
Pubmed id:2454734 Selection of a monoclonal antibody reactive with a high-molecular-weight glycoprotein circulating in the body fluid of gastrointestinal cancer patients.
Sakurai Y,Hirohashi S,Shimosato Y,Kodaira S,Abe O
Cancer Res. 1988 Jul 15;48(14):4053-8
Pubmed id:3962675 Comparison of seven cell lines derived from human gastric carcinomas.
Motoyama T,Hojo H,Watanabe H
Acta Pathol Jpn. 1986 Jan;36(1):65-83
Pubmed id:3095279 Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel.
Nakatani H,Tahara E,Yoshida T,Sakamoto H,Suzuki T,Watanabe H,Sekiguchi M,Kaneko Y,Sakurai M,Terada M
Jpn J Cancer Res. 1986 Sep;77(9):849-53

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