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pMOD-3转座子Tn5载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 pMOD-3转座子Tn5载体，带有R6K自杀型复制子，高活性19bp ME序列可被Tn5转座酶特异性识别。
The EZ-Tn5™ pMOD™ Transposon Construction Vector* was developed
for the preparation of custom EZ-Tn5 Transposons that can also be used for rescue
cloning. The vector contains a multiple cloning site (MCS) and an E. coli conditional
origin of replication (R6Kγori) flanked by the hyperactive 19-bp Mosaic Ends (ME) that
are specifically recognized by EZ-Tn5 Transposase. Also included between the ME’s are
primer binding sites for bidirectional sequencing from any custom EZ-Tn5 Transposon.
To prepare a transposon, clone any DNA sequence of interest into the MCS and then
generate the transposon either by PCR amplification using the Forward and Reverse PCR
Primers provided with the vector, or restriction enzyme digestion.
A custom EZ-Tn5 Transposon can be incubated with EZ-Tn5 Transposase in the absence
of Mg2+ to form an EZ-Tn5 Transposome™ for random insertion into the genomic DNA of
living cells.† The presence of an origin of replication enables you to propagate or rescue
the region of genomic DNA into which the transposon has been inserted. A custom
EZ-Tn5 Transposon can also be used for insertion into any target DNA in vitro*. In vitro
transposition of R6Kγori containing transposons can be used, for example, to rescue
plasmids which ordinarily do not replicate in E. coli because they lack a recognizable
origin of replication and/or a selectable marker.
The EZ-Tn5 pMOD-3 Transposon Construction Vector contains a colE1
origin of replication outside the MCS (Fig. 4). This vector works well for constructing
transposons in most cases. However, if the transposon is prepared by restriction enzyme
digestion, there is a chance that the uncut pMOD vector will interfere with downstream
applications. Replication from the R6Kγ origin is dependent on the pir gene product
produced by TransforMax™ EC100D™ pir+ and pir-116 E. coli cells which are available
separately. Since most bacterial strains do not contain a pir gene, the uncut plasmid
DNA that contaminates these transposon preparations cannot replicate and background
problems are eliminated.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
The EZ-Tn5™ pMOD™
for the preparation of custom EZ-Tn5 Transposons that can also be used for rescue
cloning. The vector contains a multiple cloning site (MCS) and an E. coli conditional
origin of replication (R6Kγori) flanked by the hyperactive 19-bp Mosaic Ends (ME) that
are specifically recognized by EZ-Tn5 Transposase. Also included between the ME’s are
primer binding sites for bidirectional sequencing from any custom EZ-Tn5 Transposon.
To prepare a transposon, clone any DNA sequence of interest into the MCS and then
generate the transposon either by PCR amplification using the Forward and Reverse PCR
Primers provided with the vector, or restriction enzyme digestion.
A custom EZ-Tn5 Transposon can be incubated with EZ-Tn5 Transposase in the absence
of Mg2+ to form an EZ-Tn5 Transposome™ for random insertion into the genomic DNA of
living cells.† The presence of an origin of replication enables you to propagate or rescue
the region of genomic DNA into which the transposon has been inserted. A custom
EZ-Tn5 Transposon can also be used for insertion into any target DNA in vitro*. In vitro
transposition of R6Kγori containing transposons can be used, for example, to rescue
plasmids which ordinarily do not replicate in E. coli because they lack a recognizable
origin of replication and/or a selectable marker.
The EZ-Tn5 pMOD-3
origin of replication outside the MCS (Fig. 4). This vector works well for constructing
transposons in most cases. However, if the transposon is prepared by restriction enzyme
digestion, there is a chance that the uncut pMOD vector will interfere with downstream
applications. Replication from the R6Kγ origin is dependent on the pir gene product
produced by TransforMax™ EC100D™ pir+ and pir-116 E. coli cells which are available
separately. Since most bacterial strains do not contain a pir gene, the uncut plasmid
DNA that contaminates these transposon preparations cannot replicate and background
problems are eliminated.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
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