E.coli S17-lambda pir strain BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli S17-lambda pir strain BioVector NTCC质粒载体菌种细胞基因保藏中心 S17-lambda pir
S17-lambda pir strain. LB stab, Storage:4℃
Organism:
Escherichia coli (Migula) Castellani and Chalmers
Designations:
S17-λpir
Genotype:
TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir.
Growth Conditions:
LB medium Temperature: 37.0°C
Applications:
transformation host
Comments:
E. coli S17-λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary).
Recovery
1.Obtain an LB agar plate with the appropriate antibiotic.
2.Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3.Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4.Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5.Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6.Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7.In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
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