OCI-AML5 cell line人急性髓系白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

OCI-AML5 cell line人急性髓系白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line:
OCI-AML5
DSMZ no.:
ACC 247
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells
Reference(s):
14482, 15051
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image

Medium:
70% alpha-MEM + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10 ng/ml GM-CSF)
Subculture:
seed out at about 0.5 x 106 cells/ml; maintain at 0.8-1.4 x 106cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; viability immediately after thawing may be rather low (<30%), but cells recover quickly and viability will raise over the next few passages. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-50 hours
Harvest:
saturation density at about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, NP
Cytogenetics:
human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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