pRL-SV40 载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRL-SV40 载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 质粒类型: 荧光素酶报告载体
启动子: SV40
克隆方法: 多克隆位点，限制性内切酶
载体大小: 3705 bp
载体抗性: Ampicillin (氨苄青霉素)
载体描述
The pRL-SV40 Vector(a,b) (Figure 1) is intended for use as an internal control reporter and may be used in combination with any experimental reporter vector to cotransfect mammalian cells. All of Promega’s pRL Reporter Vectors contain a cDNA (Rluc) encoding Renilla luciferase, which was originally cloned from the marine organism Renilla reniformis (sea pansy; 1). As described below, the Renilla luciferase cDNA(b) contained within the pRL Vectors has been modified slightly to provide greater utility.

The pRL-SV40 Vector contains the SV40 enhancer and early promoter elements to provide high-level expression of Renilla luciferase in cotransfected mammalian cells. Renilla luciferase is a 36kDa monomeric protein that does not require post-translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with a reporter vector containing the firefly luciferase gene, refer to the Dual-Luciferase ® Reporter Assay System (c,d) Technical Manual (#TM040).

The pRL Vectors are isolated from a dam–/dcm– E. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation.

The GenBank®/EMBL Accession Number for the pRL-SV40 Vector is AF025845.
质粒图谱Map：
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net