HCC1599 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCC1599 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Organism Homo sapiens, human
Tissue mammary gland; breast/duct
Cell Type epithelial, lymphoblast
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease TNM stage IIIA, grade 3, primary ductal carcinoma
Age 44 years adult
Gender female
Ethnicity Caucasian, White
Applications
HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
The cells are negative for expression of Her2-neu and for expression of p53.
The cells are poorly differentiated.
Karyotype multiploid. The cell population has several ploidy indices.
Images Cell Micrograph ATCC CRL-2331, HCC1599
Derivation
The HCC1599 breast cancer cell line was initiated on March 28, 1995, and took 10 months to establish.
Clinical Data
44 years
An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.
Caucasian, White
female
Receptor Expression
estrogen receptor, negative
progesterone receptor, negative
Oncogene her2/neu -, p53 -
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments
The tumor was classified as a TNM stage IIIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.
The cells are poorly differentiated.
The cells are negative for expression of Her2-neu and for expression of p53.
The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19.
An EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 12,13
D5S818: 12
D7S820: 10,11
THO1: 9.3
TPOX: 8
vWA: 17

Year of Origin March 28, 1998
References
Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
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