pAG31酵母Bialaphos抗性筛选标记载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAG31酵母Bialaphos抗性筛选标记载体 BioVector NTCC质粒载体菌种细胞基因保藏中心                      Vector backbone pFA6
(Search Vector Database)
Modifications to backbone Transformed E.coli cells are resistant to ampicillin. The plasmid contains the pat1 gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyl-transferase and confers resistance to the antibiotic bialaphos of transformed yeasts. Other plasmids conferring bialaphos resistance: pAG29. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. The plasmid provides the possibility for a marker rescue by homologous recombination at the direct repeats.
Vector type Yeast replicating
Selectable markers Bialaphos
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name patMX3
Species Streptomyces viridochromogenes
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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