pAG25酵母Bialaphos抗性筛选标记载体
BioVector NTCC质粒载体菌种细胞基因保藏中心

pAG25酵母Bialaphos抗性筛选标记载体
BioVector NTCC质粒载体菌种细胞基因保藏中心                    

Vector backbone pFA6
(Search Vector Database)
Modifications to backbone Transformed E.coli cells are resistant to ampicillin. The plasmid contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts. Other plasmids conferring nourseothricin resistance: pAG35, pAG36. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants.
Vector type Yeast replicating
Selectable markers Nourseothricin
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name natMX4
Species Streptomyces noursei
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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