Agrobacterium tumefaciens A136群体感应菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Agrobacterium tumefaciens A136群体感应菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Agrobacterium tumefaciens A136 A.tumefaciens A136 strain, Cat#:BioVector596668
TetR 5ug/mL,SpecR 50ug/mL.
Deposited As Agrobacterium tumefaciens (Smith and Townsend) Conn
Strain Designations A136 [At2; A136]
Isolation Derived from existing strain
Biosafety Level 1
Type Strain no
Comments Lacks the large plasmid required for virulence
Medium Nutrient broth or agar with 10 mcg/ml rifampicin
Growth Conditions Temperature: 30°C
Atmosphere: Aerobic
Isolation Derived from existing strain
References Watson B, et al. Plasmid required for virulence of Agrobacterium tumefaciens. J. Bacteriol. 123: 255-264, 1975. PubMed: 1141196

Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

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