Agrobacterium tumefaciens NTL4群体感应信号报告菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Agrobacterium tumefaciens NTL4群体感应信号报告菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Agrobacterium tumefaciens NTL4
Cat#:NTCC596666

Deposited As Agrobacterium tumefaciens
Strain Designations NTL4(pZLR4)
Application Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level 1
Type Strain no
Comments The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium LB medium with 15 mcg/ml gentamycin
Growth Conditions Temperature: 28°C
Atmosphere: Aerobic
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
References Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399

Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

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