Human herpesvirus 5 HCMV strain BioVector NTCC质粒载体菌种细胞基因保藏中心

Human herpesvirus 5 HCMV strain BioVector NTCC质粒载体菌种细胞基因保藏中心 Human herpesvirus 5
Classification Herpesviridae, Cytomegalovirus
Deposited As Cytomegalovirus
Agent Human herpesvirus 5
Strain AD-169
Common Name Human cytomegalovirus
Applications Testing virucides
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions Liquid nitrogen vapor phase
Genome Sequenced Strain Human cytomegalovirus strain AD169 complete genome
Images
Comments Preserved in virus growth medium with 7% DMSO.
GenBank: X17403.
Effect on Host CPE, cell enlarging, rounding, sloughing
Recommended Host MRC-5
Growth Conditions Temperature: 36°C
Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.5 - 2.0 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 to 1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 4 to 11 days
Effect on Host CPE, cell enlarging, rounding, sloughing
Source Adenoid tissue from 7-year-old female
References ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).
ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).
Huang L, et al. The variable e' endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037


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