Geobacillus
stearothermophilus
(ATCC® 12980 BioVector NTCC质粒载体菌种细胞基因保藏中心

Geobacillus
stearothermophilus
(ATCC® 12980 BioVector NTCC质粒载体菌种细胞基因保藏中心 Designation: NCA 26
Deposited Name: Bacillus stearothermophilus Donk
Product Description: Type strain. Produces restriction endonuclease BstPI. Used as a steam sterilization
control and sterility assurance organism.
Medium
ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Propagation Procedure
1. Open vial according to enclosed instructions.
2. From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0
mL pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a
plate.
5. Incubate the tubes and plate at 55°C for 24 hours.
Purified genomic DNA of this strain is available
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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