MIN6 Cells小鼠胰岛β细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号:MIN6 Cells小鼠胰岛β细胞
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MIN6 Cells小鼠胰岛β细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心 MIN6 Cells
Cat No.: NTCC510625
Organism Type Mouse
Tissues Default
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions, Protein Expression, Transient Transfection
Media used: DMEM low, w/ 20% cafe serum
The MIN6 cell line was derived from a mouse insulinoma and is one of a few cell lines that display characteristics of pancreatic beta-cells, including insulin secretion in response to glucose and other secretagogues. It has been reported that MIN6 cells with high passage (HP) numbers lose their ability to secrete insulin. HP MIN6 cells have gene expression changes, including downregulation of genes such as phospholipase D1 and cholecystokinin
Glucose-stimulated insulin secretion, glucose transport, glucose phosphorylation and glucose utilization have been characterized in the insulinoma cell line MIN6, which is derived from a transgenic mouse expressing the large T-antigen of SV40 in pancreatic beta cells. Glucose-stimulated insulin secretion occurred progressively from 5 mmol/l glucose, reached the maximal level approximately seven-fold above the basal level at 25 mmol/l, and remained at this level up to 50 mmol/l. Glucose transport was very rapid with the half-maximal uptake of 3-O-methyl-D-glucose being reached within 15 s at 22 degrees C. Glucose phosphorylating activity in the cell homogenate was due mainly to glucokinase; the Vmax value of glucokinase activity was estimated to be 255 +/- 37 nmol.h-1.mg protein-1, constituting approximately 80% of total phosphorylating activity, whereas hexokinase activity constituted less than 20%. MIN6 cells exhibited mainly the high Km component of glucose utilization with a Vmax of 289 +/- 18 nmol.h-1.mg protein-1. Thus, glucose utilization quantitatively and qualitatively reflected glucose phosphorylation in MIN6 cells. In contrast, MIN7 cells, which exhibited only a small increase in insulin secretion in response to glucose, had 4.7-fold greater hexokinase activity than MIN6 cells with a comparable activity of glucokinase. These characteristics of MIN6 cells are very similar to those of isolated islets, indicating that this cell line is an appropriate model for studying the mechanism of glucose-stimulated insulin secretion in pancreatic beta cells.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net [Supplier来源] http://www.biovector.net
Cat No.: NTCC510625
Organism Type Mouse
Tissues Default
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions, Protein Expression, Transient Transfection
Media used: DMEM low, w/ 20% cafe serum
The MIN6 cell line was derived from a mouse insulinoma and is one of a few cell lines that display characteristics of pancreatic beta-cells, including insulin secretion in response to glucose and other secretagogues. It has been reported that MIN6 cells with high passage (HP) numbers lose their ability to secrete insulin. HP MIN6 cells have gene expression changes, including downregulation of genes such as phospholipase D1 and cholecystokinin
Glucose-stimulated insulin secretion, glucose transport, glucose phosphorylation and glucose utilization have been characterized in the insulinoma cell line MIN6, which is derived from a transgenic mouse expressing the large T-antigen of SV40 in pancreatic beta cells. Glucose-stimulated insulin secretion occurred progressively from 5 mmol/l glucose, reached the maximal level approximately seven-fold above the basal level at 25 mmol/l, and remained at this level up to 50 mmol/l. Glucose transport was very rapid with the half-maximal uptake of 3-O-methyl-D-glucose being reached within 15 s at 22 degrees C. Glucose phosphorylating activity in the cell homogenate was due mainly to glucokinase; the Vmax value of glucokinase activity was estimated to be 255 +/- 37 nmol.h-1.mg protein-1, constituting approximately 80% of total phosphorylating activity, whereas hexokinase activity constituted less than 20%. MIN6 cells exhibited mainly the high Km component of glucose utilization with a Vmax of 289 +/- 18 nmol.h-1.mg protein-1. Thus, glucose utilization quantitatively and qualitatively reflected glucose phosphorylation in MIN6 cells. In contrast, MIN7 cells, which exhibited only a small increase in insulin secretion in response to glucose, had 4.7-fold greater hexokinase activity than MIN6 cells with a comparable activity of glucokinase. These characteristics of MIN6 cells are very similar to those of isolated islets, indicating that this cell line is an appropriate model for studying the mechanism of glucose-stimulated insulin secretion in pancreatic beta cells.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net [Supplier来源] http://www.biovector.net
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