JAWSII cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

JAWSII cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 JAWSII cell line细胞株
Cat No.: NTCC596591

Organism Mus musculus, mouse
Tissue bone marrow
Cell Type immature dendritic cell; monocyte
Product Format frozen
Morphology monocyte
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Strain C57BL/6
Storage Conditions liquid nitrogen vapor phase
Complete Growth Medium Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C

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