Enterovirus A71 (EV-A71) BrCr病毒毒蛛 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥79635
- 货 号：Enterovirus A71 (EV-A71) BrCr病毒毒蛛
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- BioVector NTCC典型培养物保藏中心
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Enterovirus A71 (EV-A71) BrCr病毒毒蛛 BioVector NTCC质粒载体菌种细胞基因保藏中心 Classification Enterovirus, Enterovirus A, Enterovirus A71 (EV-A71)
Agent Enterovirus A71 (EV-A71)
Strain BrCr
Applications
Enteric Research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Derived by passage of ATCC® VR-784™ in Vero cells (ATCC® CCL-81™). This product replaces ATCC® VR-784™ and exhibits CPE when cells are infected.
Note: The sequences of ATCC® VR-784™ and ATCC® VR-1775™ have not been compared.
Effect on Host
CPE in the form of cell rounding, sloughing and degeneration of the monolayer.
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5-9 days at 37ºC in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
CPE in the form of cell rounding, sloughing and degeneration of the monolayer.
Chain of Custody
Deposited as Enterovirus type 71
Source Stool specimen from 2-month-old male with aseptic meningitis. ® VR-784™ in Vero cells (ATCC® CCL-81™.). This product replaces ATCC® VR-784™. and exhibits CPE when cells are infected.
References
Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
Agent Enterovirus A71 (EV-A71)
Strain BrCr
Applications
Enteric Research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Derived by passage of ATCC® VR-784™ in Vero cells (ATCC® CCL-81™). This product replaces ATCC® VR-784™ and exhibits CPE when cells are infected.
Note: The sequences of ATCC® VR-784™ and ATCC® VR-1775™ have not been compared.
Effect on Host
CPE in the form of cell rounding, sloughing and degeneration of the monolayer.
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5-9 days at 37ºC in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
CPE in the form of cell rounding, sloughing and degeneration of the monolayer.
Chain of Custody
Deposited as Enterovirus type 71
Source Stool specimen from 2-month-old male with aseptic meningitis. ® VR-784™ in Vero cells (ATCC® CCL-81™.). This product replaces ATCC® VR-784™. and exhibits CPE when cells are infected.
References
Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net
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