C3H10T1/2 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

C3H10T1/2 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease sarcoma
Age embryo
Strain C3H
Applications
This line is a suitable transfection host.
Karyotype Mouse karyotype with a modal number of 80 chromosomes.
Images
Derivation
C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.
Clinical Data
The depositor recommends that the line be used between the 5th and 15th passages only.
Tumorigenic No
Effects
No, in immunosuppressed mice
RefRef
Yes, in semisolid medium
RefRef
Comments
The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.
The cells are contact sensitive.
There is no detectable background spontaneous transformation.
They are highly susceptible to transformation by chemical agents.
Tested and found negative for ectromelia virus (mousepox).
Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics.
The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.
Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.
The depositor recommends that the line be used between the 5th and 15th passages only.
Complete Growth Medium The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing
Never allow the culture to become completely confluent. Subcultivation of cultures must be performed before they reach confluence. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: Seed new flasks at 2000 viable cells/cm2.
Medium Renewal: Once between subcultures if necessary
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid notrogen vapor temperature
Culture Conditions
Temperature: 37°C
Passage History
The donor recommends that the line be used between the 5th and 15th passages only.
References
Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800
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