pAKTaq聚合酶Taq原核高表达质粒菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAKTaq聚合酶Taq原核高表达质粒菌株

BioVector NTCC质粒载体菌种细胞基因保藏中心                      

pAKTaq聚合酶Taq原核高表达质粒菌株，E.coli原核表达Taq酶。
Bacterial expression of Taq polymerase

Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

• Vector backbone
pTTQ18
• Backbone size w/o insert (bp)4563
• Vector type
Bacterial Expression
• Bacterial Resistance(s)
Ampicillin
• Growth Temperature
37°C
• Growth Strain(s)
DH5alpha
• Copy number
High Copy
• Gene/Insert name
Taq polymerase
• Species
Thermus aquaticus
• Insert Size (bp)
2501
• Cloning methodRestriction Enzyme
• 5′ cloning siteEcoRI (not destroyed)
• 3′ cloning siteBglII (unknown if destroyed)
• 5′ sequencing primerTac promoter
• 3′ sequencing primerM13_puc_F

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