EC100D pir+菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

EC100D pir+ BioVector NTCC质粒载体菌种细胞基因保藏中心                            

The TransforMax™ EC100D™ pir+ Electrocompetent E. coli and TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγori). The cells are derived from Epicentre's TransforMax™ EC100™ Electrocompetent E. coli by P1 phage transduction with a strain containing the pir+ or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker.

The TransforMax EC100D pir+ cells will maintain R6Kγori containing plasmids at approximately 15 copies per cell,1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.1

Benefits

  Accepts large clones for unbiased propagation and stability of large rescue clones.
  lacZΔM15 for blue/white screening of recombinants.
  Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
  Endonuclease minus (endA1) to ensure high yields of DNA.
  Recombination minus (recA1) for greater stability of large cloned inserts.

Genotypes

  TransforMax EC100D pir+ Electrocompetent E. coli:
  F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir+(DHFR)

  TransforMax EC100D pir-116 Electrocompetent E. coli:
  F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR)


Supplier来源:BioVector NTCC Inc.
Website网址: http://www.biovector.net