pPAGFP-C1荧光表达载体 -BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

The pPAGFP-C1 was not published. It was prepared by removing the PAGFP cDNA from pPAGFP-N1 with an AgeI/BsrGI restriction digest and subcloning it into a similarly digested pEYFP-C1 (Clontech). The pPAGFP-C1 was confirmed by sequencing. If you use this construct in future publications, use your best judgement for citation.

The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.

The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.

These plasmids are essentially the same as the Clontech pEGFP-C1 version with four mutations (L64F/T65S/V163A/T203H). The PAGFP also contains an A206K mutation to disrupt dimerization