Corynebacterium parvum strain短小棒状杆菌菌株 BioVector NTCC典型培养物保藏中心

Corynebacterium parvum

Product category

Bacteria

Strain designation

[VPI 0210]

Type strain

No

Product format

Freeze-dried

Comments

Cell wall and DNA analyses

J. Bacteriol. 109: 1047-1066, 972

Handling information

Medium

Reinforced Clostridial medium (Oxoid CM149)

Temperature

37°C

Atmosphere

Anaerobic

Handling procedure

1. Open vial according to enclosed instructions.

2. Under anaerobic conditions, withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.

3. Aseptically transfer this aliquot back into the broth.  Additional tubes of broth may be inoculated with 0.5 ml each from the suspension, and 0.1 ml may be inoculated onto a slant.  Streak several blood plates to check for colonial morphology and purity.

4. Incubate tubes under an anaerobic atmosphere at 37oC.  Incubate one agar plate anaerobically for colony formation, and one aerobically as a purity check.

5.       Within 48 hours, growth should be evident by turbidity in the broth tube with slight gas formation.  The anaerobic plate will show colonies that are pinpoint, circular, entire, convex, and smooth.  No growth should occur on agar plates incubated aerobically.

ANAEROBIC CONDITIONS:

         Anaerobic conditions for transfer may be obtained by either of the following:

·Use of an anaerobic gas chamber, or

·   Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

         Anaerobic conditions for incubation may be obtained by any of the following:

·Loose screw caps on test tubes in anaerobic chamber,

·Loose screw caps on test tubes in an activated anaerobic gas pack jar, or

·Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

【Organism物种来源】：
【Strain Designations命名】：
【Alternate State别名】：
【Type Strain模式株】Yes/No
【Isolation分离源】：
【Culture Medium培养基】：
【Cluturing培养方法】：
【Product Format发货形式】：Freeze dried
【Biosafety Level生物安全级别】：1
【Supplier生产厂家】BioVector NTCC Inc.
【网址Website】    www.biovector.net