Enterobacteria phage T7噬菌体 BioVector NTCC质粒载体菌种细胞基因保藏中心

Enterobacteria phage T7噬菌体

BioVector NTCC质粒载体菌种细胞基因保藏中心                      

Species:Enterobacteria phage T7Taxonomy:Order: CaudoviralesFamily: PodoviridaeSubfamily: AutographivirinaeType species of the genus: T7likevirusIsolate:T7NTCC No.:4623Morphology:icosahedral head, short non-contractile tailPlaques description:very large, up to 11 mm in diameter, clear centre, turbid haloImage: Single plaques on agar plate.Risk group:1Phage propagationHost bacterium:Escherichia coli (Migula 1895) Castellani and Chalmers 1919Cultivation conditions:NUTRIENT AGAR Medium , 37°C NUTRIENT AGARPeptone 5.0 gMeat extract 3.0 gAgar, if necessary 15.0 gDistilled water 1000.0 mlAdjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O isrecommended for sporulation.Phage propagation:Supply, storage and propagation of phagesRevitalisation of Vaccuum-Dried Phages
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